Mouse Anti-Human PD-1 | MyBioSource MBS690864 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human PD-1

catalog :

MBS690864

quantity :

0.1 mg

price :

475 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

(9X21)

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS690864

products type :

Antibody

products full name :

Mouse Anti-Human PD-1

products short name :

PD-1

products name syn :

PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l

other names :

programmed cell death protein 1; Programmed cell death protein 1; programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2; programmed cell death 1

products gene name :

PD-1

other gene names :

PDCD1; PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; PD1; Protein PD-1; hPD-1

uniprot entry name :

PDCD1_HUMAN

clonality :

Monoclonal

isotype :

IgG2

clone :

(9X21)

host :

Mouse

reactivity :

Human

sequence length :

288

purity :

Protein G chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

tested application :

Western Blot (WB), Neutral (N)

app notes :

Neutralization of PD-1 bioactivity: Yes. WB: Use at 1:500-1000

other info1 :

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human PD-1. Antigen: Recombinant human PD-1. Remarks: This antibody was selected for its ability to detect specifically human PD-1

other info2 :

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

products description :

Programmed Death1 (PD1) is a type I transmembrane protein belonging to the CD28/CTLA4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA4 and PD1). PD1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD1 inhibits TCRmediated T cell proliferation and production of IL1, IL4, IL10, and IFNgamma. In addition, PD1 ligation also inhibits BCR mediated signaling. PD1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Two B7 family proteins, PDL1 (also called B7H1) and PDL2 (also known as B7DC), have been identified as PD1 ligands. Unlike other B7 family proteins, both PDL1 and PDL2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens. The wide expression of PDL1 and PDL2 and the inhibitor effects on PD1 ligation indicate that PD1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases. The human PD1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immuno-globulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosinebased inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD1 signaling. Mouse and human PD1 share approximately 60% aa sequence identity.

ncbi gi num :

167857792

ncbi acc num :

NP_005009.2

ncbi gb acc num :

NM_005018.2

uniprot acc num :

Q15116

ncbi pathways :

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552); Immune System Pathway (106386); PD-1 Signaling Pathway (119557); T Cell Receptor Signaling Pathway (83080); T Cell Receptor Signaling Pathway (491)

ncbi summary :

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors. Ref.10. Subunit structure: Monomer . By similarity. Subcellular location: Membrane; Single-pass type I membrane protein. Developmental stage: Induced at programmed cell death. Involvement in disease: Systemic lupus erythematosus 2 (SLEB2) [MIM:605218]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.4. Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

size1 :

0.1 mg

price1 :

475 USD

more info or order :

MyBioSource product webpage