Rat Anti-Mouse CD105/Endoglin | MyBioSource MBS690852 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Mouse CD105/Endoglin

catalog :

MBS690852

quantity :

0.025 mg

price :

250 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MJ7/18 / IgG2a

reactivity :

mouse

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS690852

products type :

Antibody

products full name :

Rat Anti-Mouse CD105/Endoglin

products short name :

CD105/Endoglin, PE conjugated

products name syn :

Anti-mouse CD105/Endoglin-PE (#MJ7/18); Cell surface MJ7/18 antigen; CD105; Endoglin

other names :

endoglin isoform 1; Endoglin; endoglin; transmembrane glycoprotein; cell surface MJ7/18 antigen; endoglin; Cell surface MJ7/18 antigen

products gene name :

CD105

other gene names :

Eng; Eng; Endo; CD105; AI528660; AI662476; S-endoglin; Edg

uniprot entry name :

EGLN_MOUSE

clonality :

Monoclonal

isotype :

IgG2a

clone :

MJ7/18 / IgG2a

host :

Rat

reactivity :

Mouse

sequence length :

653

specificity :

The unconjugated antibody will detect native mouse CD105 in Western analysis and on the surface of mouse endothelial cells.

purity :

Protein G purified

form :

Liquid

concentration :

200 ug/ml

storage stability :

Store protected from light at 2-8 degree C. DO NOT FREEZE!

tested application :

Flow Cytometry (FC/FACS)

app notes :

The suggested use of the antibody is <=0.5ug in 100 ul volume. It is recommended that the conjugate antibody should be titrated for optimal performance for each application.

other info1 :

Immunogen: murine stromal cell line. Conjugation: PE. Preparation: Monoclonal antibodies were produced by immunizing rats with inflamed mouse skin.

other info2 :

Buffer/Stabilizer: PBS; 1% BSA. Preservative: 0,02% sodium azide. Warning: Reagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, this is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop.

products description :

Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 70-75 kDa under reducing conditions in SDS-PAGE. Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human Endoglin, the S-Endoglin and L-Endoglin that differ in the length of their cytoplasmic teails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondroytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pre-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse Endoglin share approximately 70% and 97% amino acid sequence identity in their extracellular and intracellular domains, respectively. Endoglin binds TGF-beta1 and TGF-beta3 but not TGF-beta2 efficiently by associating with TGF-beta tyme II receptor (TbetaRII). Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.

products references :

1. Cheifetz et al., J Biol Chem 267:19027, 1992 2. Parker et al., J Bone Miner Res 18:289, 2003 3. Barbara et al., J Biol Chem 274:584, 1999 4. McAllister et al., Nature Genet 8:345, 1994 5. Chen et al., Proc Natl Acad Sci 99:15468, 2002

ncbi gi num :

226437647

ncbi acc num :

NP_031958.2

ncbi gb acc num :

NM_007932.2

uniprot acc num :

Q63961

ncbi mol weight :

70,021 Da

ncbi pathways :

TGF Beta Signaling Pathway (198393); TGF-beta Receptor Signaling Pathway (198330); XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway (755427)

uniprot summary :

Function: Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells. Ref.7. Subunit structure: Homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Interacts with GDF2 . By similarity. Subcellular location: Membrane; Single-pass type I membrane protein. Tissue specificity: Endoglin is restricted to endothelial cells in all tissues except bone marrow and is also found in stromal cells within the connective tissue of intestine, stomach, heart, skeletal muscle, uterus, ovary, oviduct, testis and thymus.

size1 :

0.025 mg

price1 :

250 USD

more info or order :

MyBioSource product webpage