antibody

Rat Anti-Mouse CD3

MBS690806

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[(3B19)]

immunohistochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

Rat Anti-Mouse CD3

[CD3]

[Anti mouse CD3 (T-Cell marker)]

[CD247 antigen isoform zeta; T-cell surface glycoprotein CD3 zeta chain; CD247 antigen; CD3-zeta/eta; T-cell receptor T3 eta chain; CD3 antigen, zeta polypeptide; T-cell surface glycoprotein CD3 zeta chain; CD247 antigen; T-cell receptor T3 zeta chain]

[CD3]

[Cd247; Cd247; Cd3; T3z; Cd3h; Cd3z; Tcrk; Tcrz; Cd3-eta; Cd3zeta; AW552088; Cd3-zeta; 4930549J05Rik; A430104F18Rik; Cd3z; Tcrz]

CD3Z_MOUSE

Monoclonal

IgG2b

[(3B19)]

Rat

Mouse

164

Protein G affinity chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity.

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)

Flow cytometery: (1:100-500). Immunoprecipitation: (1:100). Immunohistochemistry (Paraffin): (1:50-400)

Testing Data

Testing Data

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with CD3 positive mouse T-Cells. IgG2b fraction of the culture supernatant was purified by Protein G affinity chromatography. Antigen: mouse CD3 positive cells

Reconstitution Buffer: PBS (sterile). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

164664500

NP_001106862.1

NM_001113391.2

P24161

Adaptive Immune System Pathway (926013); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (926019); Downstream TCR Signaling Pathway (926018); FCGR Activation Pathway (926124); Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (926123); Generation Of Second Messenger Molecules Pathway (926017); Immune System Pathway (926012); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (926043)

Function: Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. Subunit structure: Interacts with DOCK2 and TRAT1. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC . By similarity. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Ref.8 Ref.9. Subcellular location: Membrane; Single-pass type I membrane protein. Domain: The ITAM domains mediate interaction with SHB . By similarity. Post-translational modification: Phosphorylated on Tyr residues after T-cell receptor triggering. Sequence similarities: Belongs to the CD3Z/FCER1G family.Contains 3 ITAM domains.

0.1 mg

490 USD