antibody

Rat Anti-Mouse CCL-27

MBS690801

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[(6M35)]

western blot, immunohistochemistry, neutralization

Antibody

Rat Anti-Mouse CCL-27

[CCL-27]

[Ccl25; TECK; CKb15; Scya25; AI852536; A130072A22Rik]

[C-C motif chemokine 27 isoform 1; Protein Ccl27a; C-C motif chemokine 27; skinkine; skinskine; CC chemokine ILC; small inducible cytokine A27; small-inducible cytokine A27; IL-11 R-alpha-locus chemokine; small inducible cytokine A27a; chemokine (C-C motif) ligand 27; cutaneous T-cell-attracting chemokine; chemokine (C-C motif) ligand 27A]

[CCL-27]

[Ccl27a; Ccl27a; ALP; ILC; CTAK; CTACK; Ccl27; PESKY; ESkine; Scya27; Scya27a; AW558992]

A2AMS7_MOUSE

Monoclonal

IgG2

[(6M35)]

Rat

Mouse

164

This antibody detects mouse CCL-27/CTACK in Western blotting and direct ELISA

Protein G/A chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity. Avoid repeated freezing and thawing cycles. Shipping: Ships with blue ice at 4°C.

Western Blot, Immunohistochemistry, Neutralization

WB: 1:500-1000. IHC (frozen): 1:100-300. Neutralization: Yes

Antigen: Recombinant mouse CCL-27/CTACK. Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of CCL-27/CTACK.

Reconstitution Buffer: PBS (sterile). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

CCL27, also known as CTACK (cutaneous T cel-lattracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines. Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively. It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration. CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin. CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL1, and determinants on the surface of fibroblasts and endothelial cells. CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation. Endothelial cellbound CCL27 can mediate the adhesion of those cells to CLA+ T cells. CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing. In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides.

114431222

NP_001041644.1

NM_001048179.1

A2AMS7

19,495 Da

Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460)

Caution: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. Ensembl ENSMUSP00000103671 Ensembl ENSMUSP00000103672

0.1 mg

490 USD