Mouse Anti-Human Emilin-2 | MyBioSource MBS690727 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human Emilin-2

catalog :

MBS690727

quantity :

0.1 mg

price :

365 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[IgG2b/kappa; 828B3B3]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot on recombinant protein obtained from supernatant of cells transduced with an Emilin-2 adenoviral construct.

image 2 :

Immunofluorescence staining of human Emilin-2 (green) in human colon-rectal tissue (frozen section). Nuclear staining with TO-PRO3 in blue. Scale bar: 37.00 ?m.

product information

catalog number :

MBS690727

products type :

Antibody

products full name :

Mouse Anti-Human Emilin-2

products short name :

[Emilin-2]

products name syn :

[Elastin microfibril interface-loctaed protein-2]

other names :

[EMILIN-2; EMILIN-2; EMILIN-2; extracellular glycoprotein EMILIN-2; elastin microfibril interface-located protein 2; elastin microfibril interfacer 2; Elastin microfibril interface-located protein 2; Elastin microfibril interfacer 2; Protein FOAP-10]

other gene names :

[EMILIN2; EMILIN2; FOAP-10; EMILIN-2; Elastin microfibril interfacer 2]

uniprot entry name :

EMIL2_HUMAN

clonality :

Monoclonal

isotype :

IgG2, kappa

clone :

[IgG2b/kappa; 828B3B3]

host :

Mouse

reactivity :

Human

sequence length :

1053

purity :

Protein G purified

form :

Lyophilized

storage stability :

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

tested application :

Western Blot (WB). IF/IHC: IF(Frozen sections)

app notes :

Western Blot: Use at 2-5 ug/ml

image1 heading :

Western Blot

image2 heading :

Immunofluorescence

other info1 :

Immunogen: recombinant protein gC1q domain of Emilin-2. Preparation: Monoclonal antibodies were produced with the help of BALB/c mice using recombinant protein gC1q domain of Emilin-2. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

other info2 :

Buffer: PBS

products description :

Emilins (elastin microfibril interface located proteins) are extracellular matrix glycoproteins that localize to sites with proximity to elastin and microfibrils. They consist of an N-terminal cysteine-rich EMI domain and a conserved C-terminal gC1q-like domain. Emilin-1 is abundant in elastin-rich tissues such as blood vessels, skin, heart and lung. It influences placenta formation and initial organogenesis with a later role in interstitial connective tissue. Emilin-2 is larger than Emilin-1 and contains a unique proline-rich domain. It is likely involved in the process of elastogenesis. Multimerin-2 (also known as Emilin-3 or EndoGlyx-1) is expressed during embryonic development. Multimerin-1 (also known as Emilin-4) is expressed in platelets and the endothelium of blood vessels and may act as a carrier protein for platelet factor V. Emilin-5 is encoded by the Emilin-3 gene and is sometimes referred to as Emilin-3. It contains the N-terminal cysteine-rich EMI domain but lacks the C-terminal gC1q-like domain. Emilin-5 is expressed in human mesenchymal stem cells and plays an important role in skeletal development.

products references :

1. Doliana R et al, J Biol Chem 274:16773, 1999. 2. Mongiat M et al, J. Biol. Chem. 275:25471, 2000. 3. Doliana R et al, J Biol Chem 276:12003, 2001. 4. Braghetta P et al, Matrix Biol 21:603, 2002. 5. Spessotto P et al; J Biol Chem 278:6160, 2003. 6. Kishore U et al, Trends Immunol 25:551, 2004. 7. Verdone G et al, J Biomol NMR 29:91, 2004. 8. Zanetti M et al, Mol Cell Biol 24:638, 2004. 9. Mongiat et al, MCB 27:7176, 2007. 10. Mongiat et al, Neoplasia 12:294, 2010.

ncbi gi num :

60498978

ncbi acc num :

NP_114437.2

ncbi gb acc num :

NM_032048.2

uniprot acc num :

Q9BXX0

uniprot summary :

Function: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity. Subunit structure: Homotrimer associated through a moderately stable interaction of the C-terminal globular C1q domains, allowing the nucleation of the triple helix and then a further quaternary assembly to higher-order polymers via intermolecular disulfide bonds . By similarity. Interacts with EMILIN1. Subcellular location: Secreted extracellular space extracellular matrix. Note: Found mainly at the interface between amorphous elastin and microfibrils. Tissue specificity: Highest levels are present in fetal heart and adult lung. Intermediate levels in peripheral leukocytes, placenta, and spinal cord and low expression in fetal brain, spleen, thymus, and lung and in adult heart, aorta, testis, bone marrow, small intestine, thymus, lymph node, and appendix. Sequence similarities: Contains 1 C1q domain.Contains 1 collagen-like domain.Contains 1 EMI domain.

size1 :

0.1 mg

price1 :

365 USD

more info or order :

MyBioSource product webpage