antibody

Rat Anti-Mouse CCL-25

MBS690715

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[(7N28)]

western blot, immunohistochemistry, neutralization

Antibody

Rat Anti-Mouse CCL-25

[CCL-25]

[Ccl25; TECK; CKb15; Scya25; AI852536; A130072A22Rik]

[C-C motif chemokine 25; C-C motif chemokine 25; C-C motif chemokine 25; thymus-expressed chemokine; small inducible chemokine 25; small inducible cytokine A25; chemokine (C-C motif) ligand 25; Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine]

[CCL-25]

[Ccl25; Ccl25; TECK; CKb15; Scya25; AI852536; A130072A22Rik; Scya25; Teck]

CCL25_MOUSE

Monoclonal

IgG2

[(7N28)]

Rat

Mouse

This antibody detects mouse CCL-25/TECK in western blotting and direct ELISA.

Protein G/A chromatohraphy

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity. Avoid repeated freezing and thawing. Shipping: Ships with blue ice.

Western Blot (WB), Immunohistochemistry (IHC), Neutralization

Western Blot (WB): 1:500-1000. Immunohistochemistry (IHC) (frozen): 1:100-300. Neutralization: Yes

Antigen: Recombinant mouse CCL-25. Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of CCL-25.

Reconstitution Buffer: PBS (sterile). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

CCL25 (thymus expressed chemokine), also known as TECK (thymus-expressed chemokine), is a CC chemokine that is distantly related (twenty some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8.

6677881

NP_033164.1

NM_009138.3

Q3V2F3

Chemokine Receptors Bind Chemokines Pathway (926993); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (926991); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (927022); GPCR Downstream Signaling Pathway (927020); GPCR Ligand Binding Pathway (926990)

Function: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. Subcellular location: Secreted. Tissue specificity: Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine. Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

0.1 mg

490 USD