Mouse Anti-Human ALK2 (Activin R1A) | MyBioSource MBS690711 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human ALK2 (Activin R1A)

catalog :

MBS690711

quantity :

0.1 mg

price :

490 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[(9J17)]

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Human placenta(formalin fixed and paraffin embedded ) section is subjected to IHC using mouse anti-human ALK2 antibody.

product information

catalog number :

MBS690711

products type :

Antibody

products full name :

Mouse Anti-Human ALK2 (Activin R1A)

products short name :

[ALK2 (Activin R1A)]

products name syn :

[ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2]

other names :

[activin receptor type-1; activin receptor type I; hydroxyalkyl-protein kinase; activin receptor-like kinase 2; TGF-B superfamily receptor type I; activin A receptor, type II-like kinase 2; serine/threonine-protein kinase receptor R1; activin A receptor, type I]

products gene name :

[ALK2 (Activin R1A)]

other gene names :

[ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2]

clonality :

Monoclonal

isotype :

IgG1

clone :

[(9J17)]

host :

Mouse

reactivity :

Human

specificity :

This antibody was selected for its ability to detect human ALK2.

purity :

Protein G affinity chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:100-1000 (non-reducing conditions). IHC: 1:20-50

image1 heading :

Immunohistochemistry IHC

other info1 :

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human ALK2 extra cellular domain. IgG1 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. Antigen: Recombinant human ALKD2 extracellular domain.

other info2 :

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Reconstitution buffer: PBS(sterile)

products description :

Activin RIA, also known as ALK2, TSK7L, SKR1, TSRI, and ACTRI, is a glycosylated 65 kDa type I receptor in the TGFbeta serine/threonine kinase receptor family. Binding of TGFbeta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins. Activin RIA functions in a wide variety of growth and differentiation processes including gastrulation, skeletal system development, and cardiac morphogenesis. Mature human Activin RIA consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 363 aa cytoplasmic region that includes a Gly/Serrich GS box and the kinase domain. Within the ECD, human Activin RIA shares 99% and 94% aa sequence identity with mouse and rat Activin RIA, respectively. It shares 20% 26% aa sequence identity with other human type I receptors Activin RIB, BMPRIA, BMPRIB, and TGFbeta RI. BMP signaling through Activin RIA is enhanced by the direct interaction between Activin RIA and RGMB/ DRAGON, a BMP coreceptor that also associates with other type I and type II receptors. Activin RIA can additionally phosphorylate the coreceptor Endoglin and is required for the inhibitory effect of Endoglin on prostate cancer cell motility.

ncbi acc num :

NP_001096.1

uniprot acc num :

Q074771

ncbi pathways :

ALK1 Pathway (137985); ALK1 Signaling Events Pathway (137968); ALK2 Signaling Events Pathway (137983); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475)

ncbi summary :

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive. [provided by RefSeq, Jul 2008]

uniprot summary :

ALK2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. Interacts with FKBP1A. Interacts with FCHO1. Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Protein type: Protein kinase, Ser/Thr (receptor); Membrane protein, integral; EC 2.7.11.30; Kinase, protein; Protein kinase, TKL; TKL group; STKR family; Type1 subfamily. Chromosomal Location of Human Ortholog: 2q23-q24. Cellular Component: apical part of cell; integral to plasma membrane; activin receptor complex. Molecular Function: protein homodimerization activity; peptide hormone binding; metal ion binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta binding; transforming growth factor beta receptor activity, type I; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; neural crest cell migration; pharyngeal system development; in utero embryonic development; peptidyl-threonine phosphorylation; gastrulation with mouth forming second; positive regulation of bone mineralization; negative regulation of signal transduction; patterning of blood vessels; negative regulation of activin receptor signaling pathway; positive regulation of osteoblast differentiation; mesoderm formation; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of skeletal muscle development; regulation of ossification; determination of left/right symmetry; acute inflammatory response; urogenital system development; G1/S transition of mitotic cell cycle. Disease: Fibrodysplasia Ossificans Progressiva

size1 :

0.1 mg

price1 :

490 USD

more info or order :

MyBioSource product webpage