Mouse Anti-Human Podoplanin | MyBioSource MBS690680 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human Podoplanin

catalog :

MBS690680

quantity :

0.025 mg

price :

250 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[18H5]

reactivity :

human

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

FACS analysis with primary human dermal lymphatic endothelialcells (HDLEC). Upper panel: no primary antibody; Middle panel: solelyconjugated secondary antibody; Lower panel: FITC-conjugated anti-humanPodoplanin antibody.

image 2 :

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).

product information

catalog number :

MBS690680

products type :

Antibody

products full name :

Mouse Anti-Human Podoplanin

products short name :

[Podoplanin, FITC-conjugated]

products name syn :

[Anti-human Podoplanin-FITC (#18H5); Glycoprotein 36; GP36; T1-alpha; PA2.26 antigen]

other names :

[podoplanin isoform c; Podoplanin; podoplanin; T1-alpha; hT1alpha-1; hT1alpha-2; PA2.26 antigen; glycoprotein 36; glycoprotein, 36-KD; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); podoplanin; Aggrus; Glycoprotein 36; Gp36; PA2.26 antigen; T1-alpha]

other gene names :

[PDPN; PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26; GP36; Gp36; T1A]

uniprot entry name :

PDPN_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[18H5]

host :

Mouse

reactivity :

Human

sequence length :

120

specificity :

The unconjugated monoclonal antibody will detect native human Podoplanin in WB and on the surface of human LEC.

purity :

Protein G purified

form :

Liquid

concentration :

200 ug/ml

storage stability :

Store protected from light at 2-8 degree C. DO NOT FREEZE!

tested application :

Flow Cytometry (FC/FACS)

app notes :

The suggested use of the antibody is < = 0.5 ug in 100 ul volume. It is recommended that the conjugate antibody should be titrated for optimal performance for each application.

image1 heading :

Testing Data

image2 heading :

Testing Data

other info1 :

Immunogen: MDCK cells stably expressing gp36 (Podoplanin) protein. Conjugation: FITC. Preparation: Monoclonal antibodies were produced with the help of BALB/c mice using MDCK cells stably expressing gp36 (Podoplanin) protein as the immunizing antigen.

other info2 :

Buffer/Stabilizer: PBS; 1 % BSA. Preservative: 0,02 % sodium azide. Warnings: Reagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, this is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive condition may develop.

products description :

Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

products references :

1. Breiteneder-Geleff et al, Am J Pathol 154:385, 1999 2. Kerjaschki et al, Nature Med 12:230, 2006 3. Kriehuber et al, J Exp Med 194:797, 2001 4. Zimmer. et al, Biochem J 341:277, 1999 5. Kato et al, Tumour Biol 26:195, 2005 6. Katsue-Inoue et al, J Biol Chem 282:25993, 2007 7. Kato et al, Oncogene 23:8552, 2004 8. Schacht. et al, Am J Pathol 166:913, 2005 9. Mishima et al, Acta Neuropathol 111:483, 2006

ncbi gi num :

54792060

ncbi acc num :

NP_001006625.1

ncbi gb acc num :

NM_001006624.1

uniprot acc num :

Q86YL7

ncbi summary :

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. Ref.1 Ref.6 Ref.10 Ref.11 UniProtKB Q62011. Subcellular location: Membrane; Single-pass type I membrane protein . By similarity. Cell projection filopodium membrane; Single-pass type I membrane protein . By similarity. Cell projection lamellipodium membrane; Single-pass type I membrane protein . By similarity. Cell projection microvillus membrane; Single-pass type I membrane protein . By similarity. Cell projection ruffle membrane; Single-pass type I membrane protein . By similarity. Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles . By similarity. UniProtKB Q64294 UniProtKB Q62011. Tissue specificity: Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. Ref.2 Ref.6 Ref.10. Post-translational modification: Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Ref.2 Ref.6 Ref.11The N-terminus is blocked . By similarity. UniProtKB Q64294. Sequence similarities: Belongs to the podoplanin family. Sequence caution: The sequence AAM73655.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAO22143.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAC11550.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAC11557.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAG35495.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

size1 :

0.025 mg

price1 :

250 USD

more info or order :

MyBioSource product webpage