antibody

Rat Anti-Mouse CD103

MBS690673

0.1 mg

475 USD

monoclonal

rat

nonconjugated

(7A3)

immunohistochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

Antibody

Rat Anti-Mouse CD103

CD103

Anti mouse CD103 (#7A3)

integrin alpha-E isoform 1; Integrin alpha-E; integrin alpha-E; integrin alpha M290; integrin alpha E, epithelial-associated; Integrin alpha M290

CD103

Itgae; Itgae; CD103; aM290; alpha-E1; A530055J10; alpha-M290

ITAE_MOUSE

Monoclonal

IgG2

(7A3)

Rat

Mouse

1167

Protein A/G affinity chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Immunohistochemistry (IHC) - Paraffin, Flow Cytometry (FC/FACS)

1. FACS analysis: Yes (1:500-2000) 2. Immunohistochemistry (Paraffin): Yes (1:50-200)

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with Mouse mammary tumor cells. IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. Antigen: Mouse mammary tumor cells

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Remarks: This antibody was selected for its ability to detect mouse CD103.

Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.

226823202

NP_032425.2

NM_008399.2

Q60677

Extracellular Matrix Organization Pathway (927061); Focal Adhesion Pathway (198353); Integrin Cell Surface Interactions Pathway (927077); Integrin-mediated Cell Adhesion Pathway (198372); Regulation Of Actin Cytoskeleton Pathway (83286); Regulation Of Actin Cytoskeleton Pathway (500)

Function: Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Mice expressing a null mutation of the alpha-E subunit gene exhibit a marked reduction in the numbers of intraepithelial lymphocytes in the gut and in the development of gut-associated lymphoid aggregates, supporting a specific role for this integrin in mediating retention of lymphocytes in the intestinal wall. Subunit structure: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chains linked by a disulfide bond. Alpha-E associates with beta-7. Subcellular location: Membrane; Single-pass type I membrane protein. Domain: The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. Sequence similarities: Belongs to the integrin alpha chain family.Contains 7 FG-GAP repeats.Contains 1 VWFA domain.

0.1 mg

475 USD