Mouse Anti-Human Activin R2A | MyBioSource MBS690639 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human Activin R2A

catalog :

MBS690639

quantity :

0.1 mg

price :

475 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

(6J16)

reactivity :

human

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS690639

products type :

Antibody

products full name :

Mouse Anti-Human Activin R2A

products short name :

Activin R2A

products name syn :

Anti human Activin R-2A (#6J16)

other names :

activin receptor type-2A isoform 1; Activin receptor type-2A; activin receptor type-2A; activin A receptor, type IIA; Activin receptor type IIA

products gene name :

Activin R2A

other gene names :

ACVR2A; ACVR2A; ACVR2; ACTRII; ACVR2; ACTR-IIA; ACTRIIA

uniprot entry name :

AVR2A_HUMAN

clonality :

Monoclonal

isotype :

IgG2

clone :

(6J16)

host :

Mouse

reactivity :

Human

sequence length :

513

specificity :

This antibody was selected for its ability to detect human Activin RIIA. No cross reactivity was found to hActivin RI and RIIB.

purity :

Protein G affinity chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500 – 1:1000. IHC (Paraffin): 1:50 – 1:100

other info1 :

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human Activin receptor IIA extra cellular domain. IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

other info2 :

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Protein RefSeq: NP_001607.1. mRNA RefSeq: NM_001616.3. Antigen: recombinant human Activin receptor IIA extracellular domain

products description :

Activin proteins are involved in a wide range of biological processes including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, the regulation of reproductive physiology, inflammation, and carcinogenesis. They function through heteromeric complexes of type I and type II serine/threonine kinase receptors. Dimeric ligands bind to a type II receptor, such as Activin Receptor IIA (ActRIIA or activin R2A), which then associates with a type I receptor to initiate signal transduction. ActRIIA mediates the pleiotropic effects of Activins and Inhibins as well as several members of the BMP and GDF families of TGF beta like proteins. Mature human ActRIIA is a 70 kDa glycoprotein that consists of a 116 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 352 aa cytoplasmic region that includes the kinase domain and a PDZbinding motif. Within the ECD, human ActRIIA shares 98% aa sequence identity with mouse and rat ActRIIA. Signaling through ActRIIA is modulated by its interaction with RGMB/ DRAGON, Cripto, Endoglin/CD105, TGFbeta RIII/Betaglycan, or BAMBI. These interactions can enhance ligandinduced signaling or interfere with signaling by preventing ActRIIA association with type I receptors. Activininduced responses can also be limited by the enhanced internalization of ActRIIA following its association with the cytoplasmic proteins ARIP1 and ARIP2.

ncbi gi num :

518828583

ncbi acc num :

NP_001265508.1

ncbi gb acc num :

NM_001278579.1

uniprot acc num :

P27037

ncbi mol weight :

57,848 Da

ncbi pathways :

ALK1 Signaling Events Pathway (137968); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); Regulation Of Signaling By NODAL Pathway (477131); Signal Transduction Pathway (477114); Signaling By Activin Pathway (730349); Signaling By BMP Pathway (106336); Signaling By NODAL Pathway (477130); TGF-beta Signaling Pathway (83064)

ncbi summary :

This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jun 2013]

uniprot summary :

Function: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. Catalytic activity: ATP + [receptor-protein] = ADP + [receptor-protein] phosphate. Cofactor: Magnesium or manganese . By similarity. Subunit structure: Interacts with AIP1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3 . By similarity. Subcellular location: Membrane; Single-pass type I membrane protein. Sequence similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.Contains 1 protein kinase domain.

size1 :

0.1 mg

price1 :

475 USD

more info or order :

MyBioSource product webpage