Hamster Anti-Mouse CD80 | MyBioSource MBS690572 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Hamster Anti-Mouse CD80

catalog :

MBS690572

quantity :

0.1 mg

price :

490 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

[(MAB0713)]

reactivity :

mouse

application :

immunohistochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of CD80 using MBS690572 (Left panel: Red Pulp, Right panel: White Pulp)

image 2 :

Formalin fixed and paraffin embedded mouse tumor section was subjected to IHC staining of CD80 using MBS690572 (Left panel:x 20; Right panel: x 40)

product information

catalog number :

MBS690572

products type :

Antibody

products full name :

Hamster Anti-Mouse CD80

products short name :

[CD80]

products name syn :

[Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17]

other names :

[T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen; CD80 antigen; Activation B7-1 antigen]

products gene name :

[CD80]

other gene names :

[Cd80; Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17; B7; B7]

uniprot entry name :

CD80_MOUSE

clonality :

Monoclonal

isotype :

IgG

clone :

[(MAB0713)]

host :

Armenian Hamster

reactivity :

Mouse

sequence length :

306

purity :

Protein G/A chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

tested application :

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

IHC (paraffine): 1:100-1:300. Flow cytometry: 1:100-1:1000

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a Armenian Hamster immunized with recombinant Mouse CD80 protein. IgG fraction of the culture supernatant was purified by Protein G affinity chromatography. Reconstitution buffer: PBS(sterile). Antigen: Recombinant Mouse CD80 protein

other info2 :

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Remarks: This antibody was selected for its ability to detect mouse CD80.

products description :

B7-1 (CD80) and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the downregulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be upregulated through interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily.

ncbi gi num :

112983684

ncbi acc num :

NP_033985.3

ncbi gb acc num :

NM_009855.2

uniprot acc num :

Q00609

ncbi pathways :

Adaptive Immune System Pathway (926013); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (926020); CD28 Dependent PI3K/Akt Signaling Pathway (926021); CD28 Dependent Vav1 Pathway (926022); CTLA4 Inhibitory Signaling Pathway (926023); Cell Adhesion Molecules (CAMs) Pathway (83266)

uniprot summary :

Function: Involved in the costimulatory signal essential for T lymphocytes activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Subcellular location: Membrane; Single-pass type I membrane protein. Tissue specificity: Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies. Developmental stage: Expressed between 4 and 12 hours post-activation. Protein was detected at cell surface at 24 hours and it's expression was maximal from 48 to 72 hours post-activation. Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.

size1 :

0.1 mg

price1 :

490 USD

more info or order :

MyBioSource product webpage