antibody

Anti-Human Cystatin SN

MBS690498

0.1 mg

490 USD

monoclonal

mouse

nonconjugated

[(5N51)]

Antibody

Anti-Human Cystatin SN

[Cystatin SN]

[CST1]

[cystatin-SN; Cystatin-SN; cystatin-SN; cystatin 1; cystatin-1; cystain-SA-I; cystatin SA-I; salivary cystatin-SA-1; cysteine proteinase inhibitor, type 2 family; cystatin SN; Cystain-SA-I; Cystatin-1; Salivary cystatin-SA-1]

[CST1; CST1]

CYTN_HUMAN

Monoclonal

IgG2

[(5N51)]

Mouse

Human

141

This antibody was selected for its ability to detect human Cystatin-SN

Protein G chromatography

Lyophilized

Ships at 4°C. Upon receipt lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at <-20°C for at least for six months without detectable loss of activity.

Western Blot (WB)

WB: 1:500-1000. Optimal dilutions should be determined by each laboratory for each application!

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant Cystatin-SN.

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Reconstitution Buffer: PBS (sterile). Antigen: Human Recombinant Cystasin-SN

Cystatin SN is a member of family 2 of the cystatin superfamily. Together with Cystatins S and SA, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva. Cystatin SN inhibits members of the papain family including Cathepsins B, C, H and L.

19882251

NP_001889.2

NM_001898.2

P01037

Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid. [provided by RefSeq, Jul 2008]

Function: Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well. Subcellular location: Secreted Ref.10. Tissue specificity: Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid. Ref.10. Sequence similarities: Belongs to the cystatin family. Mass spectrometry: Molecular mass is 14303.1553 0.0675 Da from positions 21 - 141. Determined by ESI. Ref.10Molecular mass is 14319.171 0.0523 Da from positions 21 - 141. Determined by ESI. Variant Leu-31. Ref.10

0.1 mg

490 USD