antibody

Mouse Anti-Human IL-1 R2

MBS690482

0.1 mg

475 USD

monoclonal

mouse

nonconjugated

(4T28)

western blot, immunohistochemistry, neutralization

Antibody

Mouse Anti-Human IL-1 R2

IL-1 R2

Il1r2; CD121b; Il1r-2; Interleukin 1 receptor 2

interleukin-1 receptor type 2 isoform 2; Interleukin-1 receptor type 2; interleukin-1 receptor type 2; IL-1R-beta; antigen CDw121b; IL-1 type II receptor; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; type II interleukin-1 receptor, beta; interleukin 1 receptor type II variant 3; interleukin 1 receptor, type II; CD121 antigen-like family member B; CDw121b; IL-1 type II receptor; Interleukin-1 receptor beta; IL-1R-beta; Interleukin-1 receptor type II

IL-1 R2

IL1R2; IL1R2; IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2; IL1RB; IL-1R-2; IL-1RT-2; IL-1RT2; IL-1R-beta; mIL-1R2; mIL-1RII; sIL-1R2; sIL-1RII

IL1R2_HUMAN

Monoclonal

IgG2

(4T28)

Mouse

Human

296

Protein G chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Shipping: 4 degree C

Western Blot (WB), Immunohistochemistry (IHC) - Formalin, Neutralization

WB: 1:500-1:2000. IHC-F: 1:20-1:200. Nuetralization of IL-1 RII mediated bioactivity: Yes

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of IL-1 R II extracellular domain. Antigen: Recombinant human IL1-R II EC domain.

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Reconstitution Buffer: PBS (sterile)

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and, endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. IL-1 RII has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

387527984

NP_001248348.1

NM_001261419.1

P27930

45,421 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Modulation And Signaling Pathway (198822); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage. [provided by RefSeq, May 2012]

Function: Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Ref.8 Ref.11 Ref.12 Ref.13. Subunit structure: Associates with IL1RAP to form a non-signaling interleukin-1 receptor complex. Ref.14. Subcellular location: Isoform Short: Secreted Ref.2. Isoform Long: Cell membrane; Single-pass type I membrane protein Ref.2. Post-translational modification: A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase; hovever, several sIL1R2 forms ranging from 45 and 60 kDa are reported. Sequence similarities: Belongs to the interleukin-1 receptor family.Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

0.1 mg

475 USD