antibody

Rat Anti-Mouse Klotho

MBS690455

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[(4F54)]

western blot, immunohistochemistry

Antibody

Rat Anti-Mouse Klotho

[Klotho]

[Anti-mouse Klotho (#4F54)]

[klotho; Klotho; klotho; secreted form of Klotho protein; klotho]

[Kl; Kl; alpha-kl]

KLOT_MOUSE

Monoclonal

IgG2

[(4F54)]

Rat

Mouse

1014

This antibody detects mouse Klotho in Western blotting.

Protein A/G chromatography

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Immunohistochemistry (IHC) - Formalin, Western Blot (WB)

Western blot: 1:500-1000 . IHC (Frozen): 1:50-200

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant protein of Klotho. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. Antigen: Recombinant mouse Klotho

Reconstitution buffer: PBS (sterile). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

The Klotho proteins, Klotho and Klotho beta (beta KL), are type I transmembrane glycoproteins with extracellular regions that contain two beta-glycosidase-like repeats. Klotho has both membrane-bound and soluble forms; the soluble form predominates and consists of only the first beta-glycosidase-like repeat. Klotho has beta-glucuronidase activity against select steroid beta-glucuronides, appears to regulate calcium and vitamin D levels in serum, and is associated with premature aging. Klotho beta, a divergent structural member of the glycosidase I superfamily, is primarily expressed in the liver and pancreas, with lower expression in adipose tissue. Klotho beta mediates the interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver, which negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes. Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes. The expression pattern of Klotho beta suggests it may play a role in lipid metabolism.

227330584

NP_038851.2

NM_013823.2

O35082

Adaptive Immune System Pathway (926013); Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (421792); Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (468309); Constitutive PI3K/AKT Signaling In Cancer Pathway (926316); DAP12 Interactions Pathway (926127); DAP12 Signaling Pathway (926128); Disease Pathway (926229); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (926027); Downstream Signal Transduction Pathway (926957); Downstream Signaling Of Activated FGFR Pathway (926257)

Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15The Klotho peptide generated by cleavage of the membrane-bound isoform maybe an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. Ref.1 Ref.5 Ref.6 Ref.9 Ref.14 Ref.15. Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol. Ref.13 Ref.14. Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid. Ref.13. Subunit structure: Homodimer. Interacts with FGF23 and FGFR1. Ref.12 Ref.15. Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: Isoform 1 shedding leads to a soluble peptide. Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14Isoform 2: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14. Klotho peptide: Secreted Ref.1 Ref.4 Ref.8 Ref.11 Ref.12 Ref.14. Tissue specificity: Membrane-bound protein is present in distal renal tubules, inner ear, ependymal cells of brain choroid plexus, elongating spermatids and mature oocytes (at protein level). Soluble peptide is present in serum (100 pM) and cerebrospinal fluid. Expressed strongly in kidney, moderately in brain choroid plexus, and at low levels in pituitary, placenta, skeletal muscle, urinary bladder, aorta, pancreas, testis, ovary, colon, thyroid gland and adipocytes. Ref.1 Ref.2 Ref.4 Ref.7 Ref.8 Ref.11 Ref.12 Ref.14. Developmental stage: Not expressed in the embryo. Expressed in the kidney of newborns. Ref.1. Induction: Induced by 1,25-dihydroxyvitamin D3 in kidney. Down-regulated by angiotensin II and up-regulated by statins through modulation of the RhoA pathway in epithelial cells (in vitro). Isoform 1 (but not isoform 2)is up-regulated by thyroid hormone in adipocytes. Ref.7 Ref.9 Ref.10 Ref.13. Domain: Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 241, and the second one lacks the essential Glu active site residue at position 874. Post-translational modification: N-glycosylated. Ref.12. Disruption phenotype: Mice display a syndrome resembling to human aging, with short lifespan, infertility, atherosclerosis, skin atrophy, osteoporosis and emphysema. They have various metabolic abnormalities, including increased insulin sensitivity and decreased insulin production. Mice overexpressing Kl have increased resistance to insulin and IGF1, a lifespan extended of more than 20%, and generate fewer offspring. Ref.5. Sequence similarities: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. Biophysicochemical propertiesKinetic parameters:KM=0.249 mM for 4-methylumbelliferylglucuronide Ref.13KM=0.251 mM for estrone 3-beta-D-glucuronideKM=0.174 mM for beta-estradiol 3-beta-D-glucuronideKM=0.251 mM for estriol 3-beta-D-glucuronideVmax=0.62 uM/h/ug enzymepH dependence:Optimum pH is 5.5.

0.1 mg

490 USD