antibody

Rat Anti-Human CD26

MBS690414

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[1G24]

western blot, flow cytometry, FACS

Antibody

Rat Anti-Human CD26

[CD26]

[DPP4; CD26; ADABP; ADCP2; DPPIV; TP103]

[dipeptidyl peptidase 4; Dipeptidyl peptidase 4; dipeptidyl peptidase 4; ADCP-2; DPP IV; dipeptidylpeptidase 4; dipeptidyl peptidase IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); dipeptidyl-peptidase 4; ADABP; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; TP103; CD_antigen: CD26Cleaved into the following 2 chains:Dipeptidyl peptidase 4 membrane formAlternative name(s):Dipeptidyl peptidase IV membrane form]

[CD26]

[DPP4; DPP4; CD26; ADABP; ADCP2; DPPIV; TP103; ADCP2; CD26; ADCP-2; DPP IV]

DPP4_HUMAN

Monoclonal

IgG2

[1G24]

Rat

Human

766

Protein G chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Avoid repeated freeze adn thaw cycles.

Western Blot (WB), Flow Cytometry (FC/FACS)

Western Blot: 1:500 - 1:1000; Flow cytometry: 1:50 - 1:200

Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant CD26 (also called Dipeptidyl peptidase IV (DPPIV)) extracellular domain.

Antigen: Human recombinant CD26 EC domain. Reconstitution: PBS (sterile). Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Remark: This antibody was selected for its ability to detect human CD26 protein.

Dipeptidyl peptidase IV (DPPIV/CD26) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of proteins and peptides. It plays an important role in T-cell costimulation, chemokine biology, type II diabetes and tumor biology. There are at least 15 DPPIV-related human genes. Some function as enzymes, including DPP8, DPP9, fibroblast activation protein (FAP), prolyl endopeptidase (PREP), and N-acylaminoacyl-peptide hydrolase (APEH) while others (DPP6/DPPX and DPP10/DPRP3) may have lost their protease properties but acquired novel functions. For example, DPP6 is a critical component of neuronal A-type K+ channels.

18765694

NP_001993.2

NM_001935.3

P27487

88,279 Da

Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Synthesis, Secretion, And Inactivation Of Glucagon-like Peptide-1 (GLP-1) Pathway (187171); Synthesis, Secretion, And Inactivation Of Glucose-dependent Insulinotropic Polypeptide (GIP) Pathway (187172)

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

Function: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Ref.13 Ref.14 Ref.20 Ref.23 Ref.24 Ref.28 Ref.30 Ref.31 Ref.32. Catalytic activity: Release of an N-terminal dipeptide, Xaa-Yaa- -Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline. Enzyme regulation: Inhibited by GPC3 and diprotin A. Ref.14 Ref.32. Subunit structure: Monomer. Homodimer or heterodimer with Seprase (FAP). Requires homodimerization for optimal dipeptidyl peptidase activity and T-cell costimulation. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Associates with collagen. Interacts with PTPRC; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Interacts (extracellular domain) with ADA; does not inhibit its dipeptidyl peptidase activity. Interacts with CAV1 (via the N-terminus); the interaction is direct. Interacts (via cytoplasmic tail) with CARD11 (via PDZ domain); its homodimerization is necessary for interaction with CARD11. Interacts with IGF2R; the interaction is direct. Interacts with GPC3. Interacts with human coronavirus-EMC spike protein and acts as a receptor for this virus. Ref.13 Ref.14 Ref.16 Ref.17 Ref.23 Ref.26 Ref.27 Ref.28 Ref.30 Ref.31 Ref.36 Ref.37 Ref.38 Ref.39 Ref.40. Subcellular location: Dipeptidyl peptidase 4 soluble form: Secreted. Note: Detected in the serum and the seminal fluid. Ref.13 Ref.16 Ref.23 Ref.24 Ref.25 Ref.26 Ref.30 Ref.31Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Cell projection invadopodium membrane; Single-pass type II membrane protein. Cell projection lamellipodium membrane; Single-pass type II membrane protein. Cell junction. Membrane raft. Note: Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface. Ref.13 Ref.16 Ref.23 Ref.24 Ref.25 Ref.26 Ref.30 Ref.31. Tissue specificity: Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. Ref.12 Ref.32. Induction: Up-regulated by IL12/interleukin-12 on activated T-cells. IL12-activated cells expressed enhanced levels of DPP4 but not mRNAs. Down-regulated by TNF. Up-regulated in migratory endothelial cells and in the invasive endothelial cells in tumors. Ref.14 Ref.19 Ref.22 Ref.26 Ref.30 Ref.32. Domain: The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. Post-translational modification: The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.N- and O-Glycosylated. Ref.23 Ref.25 Ref.38 Ref.39 Ref.40 Ref.41Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. Ref.23. Miscellaneous: Level of plasma concentrations of the soluble form (SDPP) can be managed as a colon carcinoma diagnostic and prognostic marker. Sequence similarities: Belongs to the peptidase S9B family. DPPIV subfamily.

0.1 mg

490 USD