antibody

Rat Anti-Mouse CCL-4

MBS690409

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[(5G13)]

ELISA, neutralization, enzyme immunoassay

Antibody

Rat Anti-Mouse CCL-4

[CCL-4]

[Ccl4; Act-2; Mip1b; Scya4; MIP-1B; AT744.1]

[C-C motif chemokine 4; C-C motif chemokine 4; C-C motif chemokine 4; ACT2; SIS-gamma; MIP-1 beta; MIP-1-beta; immune activation protein 2; small inducible cytokine A4; small-inducible cytokine A4; macrophage inflammatory protein 1-beta; chemokine (C-C motif) ligand 4; Immune activation protein 2; ACT-2; ACT2; Macrophage inflammatory protein 1-beta; MIP-1-beta; Protein H400; SIS-gamma; Small-inducible cytokine A4]

[CCL-4]

[Ccl4; Ccl4; Act-2; Mip1b; Scya4; MIP-1B; AT744.1; Mip1b; Scya4; ACT-2; ACT2; MIP-1-beta]

CCL4_MOUSE

Monoclonal

IgG2

[(5G13)]

Rat

Mouse

92

This antibody detects mouse CCL-4 in direct ELISA

Protein G/A chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity. Avoid repeated freezing and thawing. Ships with blue ice at 4°C.

ELISA (EIA), Neutralization

ELISA: Yes. Neutralization: Yes

Preparation: This antibody was produced from hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of CCL-4. Antigen: Recombinant mouse CCL-4

Reconstitution buffer: PBS (sterile). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

CCL4, also known as macrophage inflammatory protein 1 beta (MIP1beta), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP1alpha. The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV. Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of Mtropic HIV1 which utilizes CCR5 as a coreceptor. Both forms of CCL4 block HIV1 infection of T cells by inducing the downregulation of CCR5. Mature mouse CCL4 shares 77% and 86% amino acid sequence identity with human and rat CCL4, respectively.

7305459

NP_038680.1

NM_013652.2

P14097

Chemokine Receptors Bind Chemokines Pathway (926993); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (926991); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125146); Cytosolic DNA-sensing Pathway (124833); GPCR Ligand Binding Pathway (926990)

Function: Monokine with inflammatory and chemokinetic properties. Subunit structure: Homodimer . By similarity. Subcellular location: Secreted. Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

0.1 mg

490 USD