Rat Anti-Mouse CD45R (B220) | MyBioSource MBS690362 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Anti-Mouse CD45R (B220)

catalog :

MBS690362

quantity :

0.1 mg

price :

475 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

(9F14)

reactivity :

mouse

application :

immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS690362

products type :

Antibody

products full name :

Rat Anti-Mouse CD45R (B220)

products short name :

CD45 receptor (B220)

products name syn :

Anti mouse CD45R (B220) (#9F14)

other names :

Receptor-type tyrosine-protein phosphatase C; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; lymphocyte antigen 5; leukocyte common antigen; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; Lymphocyte antigen 5; Ly-5; T200

products gene name :

CD45 receptor (B220)

other gene names :

Ptprc; Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4; Ly-5; L-CA; Ly-5

uniprot entry name :

PTPRC_MOUSE

clonality :

Monoclonal

isotype :

IgG2

clone :

(9F14)

host :

Rat

reactivity :

Mouse

sequence length :

1291

specificity :

CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

purity :

Protein G/A affinity chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Shipping: Ships on coldpacks

tested application :

IHC (P): Immunohistochemistry (paraffin). FC: Flow Cytometry

app notes :

Flow cytometry: 1:100-1:400. IHC (paraffin): 1:50-1:200

other info1 :

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse Leukemia virus-induced pre-B tumor cells. IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. Antigen: Leukemia virus-induced pre-B tumor cells.

other info2 :

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Reconstitution Buffer: PBS (Sterile). Target: B220. Remarks: This antibody was selected for its ability to detect mouse CD45R

products description :

CD45R is an isoform of CD45, also known as B220. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, subsets of T and NK cells.

ncbi gi num :

341942176

ncbi acc num :

NP_001104786.2

uniprot acc num :

P06800

ncbi pathways :

Adaptive Immune System Pathway (926013); Axon Guidance Pathway (926383); B Cell Receptor Signaling Pathway (198285); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (926382); EPO Receptor Signaling Pathway (198317); Fc Gamma R-mediated Phagocytosis Pathway (114297); Fc Gamma R-mediated Phagocytosis Pathway (106590); Immune System Pathway (926012)

uniprot summary :

Function: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN . By similarity. Dephosphorylates LYN, and thereby modulates LYN activity. Ref.14. Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Subunit structure: Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes . By similarity. Binds GANAB and PRKCSH. Ref.13. Subcellular location: Membrane; Single-pass type I membrane protein. Membrane raft . By similarity. Note: Colocalized with DPP4 in membrane rafts . By similarity. Developmental stage: Expression is restricted to the hematopoietic compartment of development. Domain: The first PTPase domain interacts with SKAP1 . By similarity. Post-translational modification: Heavily N- and O-glycosylated. Sequence similarities: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.Contains 2 fibronectin type-III domains.Contains 2 tyrosine-protein phosphatase domains. Sequence caution: The sequence AAA39462.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

size1 :

0.1 mg

price1 :

475 USD

more info or order :

MyBioSource product webpage