antibody

Mouse Anti-Human IL-1 beta

MBS690357

0.1 mg

490 USD

monoclonal

mouse

nonconjugated

[(4M27)]

western blot, immunohistochemistry, neutralization

Antibody

Mouse Anti-Human IL-1 beta

[IL-1 beta]

[IL1B; IL-1; IL1F2; IL1-BETA]

[interleukin-1 beta proprotein; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1, beta; Catabolin]

[IL-1 beta]

[IL1B; IL1B; IL-1; IL1F2; IL1-BETA; IL1F2; IL-1 beta]

IL1B_HUMAN

Monoclonal

IgG2

[(4M27)]

Mouse

Human

269

Protein G chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at <-20° C for at least for six months without detectable loss of activity.

Western Blot (WB), Immunohistochemistry (IHC) - Formalin, Neutralization.

Western Blot: 1:500-1:2000. IHC (frozen) 1:20-1:100. Neutralization: Yes

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of IL-1b. Antigen: Recombinant human IL-1 beta. Reconstitution buffer: PBS (sterile).

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Remarks: This antibody was selected for its ability to detect IL-1 beta.

IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.

10835145

NP_000567.1

NM_000576.2

P01584

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Ref.16. Subunit structure: Monomer. Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. Sequence similarities: Belongs to the IL-1 family.

0.1 mg

490 USD