Hamster Anti-Mouse CD40 Ligand | MyBioSource MBS690292 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Hamster Anti-Mouse CD40 Ligand

catalog :

MBS690292

quantity :

0.1 mg

price :

490 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

[(MAB0712)]

reactivity :

mouse

application :

immunohistochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of CD40 Ligand using MBS690292 (Left panel Red Pulp, Right panel: White Pulp).

image 2 :

Formalin fixed and paraffin embedded mouse tumor section was subjected to IHC staining of CD40 Ligand using MBS690292 (Left panel: x 20; Right panel: x 40).

product information

catalog number :

MBS690292

products type :

Antibody

products full name :

Hamster Anti-Mouse CD40 Ligand

products short name :

[CD40 Ligand]

products name syn :

[Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5]

other names :

[CD40 ligand; CD40 ligand; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5]

products gene name :

[CD40 Ligand]

other gene names :

[Cd40lg; Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Cd40l; Tnfsf5; CD40-L; TRAP]

uniprot entry name :

CD40L_MOUSE

clonality :

Monoclonal

isotype :

IgG2

clone :

[(MAB0712)]

host :

Hamster

reactivity :

Mouse

sequence length :

260

purity :

Protein A/G affinity chromatography

form :

lyophilized

storage stability :

Stability: Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Ships Room Temperature or 4 degree C.

tested application :

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

app notes :

IHC (paraffine): 1:100-1:300. Flow cytometry: 1:100-1:1000. This antibody was selected for its ability to detect mouse CD40 Ligand.

image1 heading :

Immunohistochemistry

image2 heading :

Immunohistochemistry

other info1 :

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a Armenian Hamster) immunized with mouse Th1 cells. IgG2 fraction of the culture supernatant was purified by Protein G affinity chromatography.

other info2 :

Reconstitution: Reconstitute the antibody with 400 ul sterile PBS and the final concentration is 500 ug/ml. Antigen: Mouse Th1 cells . Reconstitution buffer: PBS (sterile)

products description :

CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site. CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78% amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes. Both membrane bound and soluble CD40L induce similar effects on B cells.

ncbi gi num :

15011846

ncbi acc num :

NP_035746.2

ncbi gb acc num :

NM_011616.2

uniprot acc num :

P27548

ncbi mol weight :

29,370 Da

ncbi pathways :

Adaptive Immune System Pathway (926013); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Asthma Pathway (83313); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine-cytokine Receptor Interaction Pathway (83248)

uniprot summary :

Function: Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching . By similarity. Subunit structure: Homotrimer. Subcellular location: Cell membrane; Single-pass type II membrane protein. CD40 ligand, soluble form: Secreted. Tissue specificity: Specifically expressed on activated CD4+ T-lymphocytes. Post-translational modification: The soluble form derives from the membrane form by proteolytic processing . By similarity. Sequence similarities: Belongs to the tumor necrosis factor family.

size1 :

0.1 mg

price1 :

490 USD

more info or order :

MyBioSource product webpage