Mouse Anti-Human TNFSF10 | MyBioSource MBS690229 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human TNFSF10

catalog :

MBS690229

quantity :

0.1 mg

price :

475 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

(6D44)

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS690229

products type :

Antibody

products full name :

Mouse Anti-Human TNFSF10

products short name :

TNFSF10

products name syn :

TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L

other names :

tumor necrosis factor ligand superfamily member 10 isoform 2; Tumor necrosis factor ligand superfamily member 10; tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor (ligand) superfamily, member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand

products gene name :

TNFSF10

other gene names :

TNFSF10; TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; APO2L; TRAIL; Apo-2L

uniprot entry name :

TNF10_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

(6D44)

host :

Mouse

reactivity :

Human

sequence length :

101

purity :

Protein G chromatography

form :

Lyophilized

storage stability :

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

tested application :

Western Blot (WB), Immunohistochemistry (IHC) - Paraffin, Flow Cytometry (FC/FACS)

app notes :

IHC (paraffine): 1:20 - 1:100. WB: Use at 1:500-1000. Flow cytometry: 1:50 - 1:200

other info1 :

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with Human TNFSF10 recombinant protein (also called TRAIL). Antigen: recombinant human TNFSF10

other info2 :

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Reconstitution buffer: PBS (sterile). Remarks: This antibody was selected for its ability to detect human TNFSF10. Database References Target: Protein RefSeq: NP_00380.1. mRNA RefSeq: NM_003810.3

products description :

TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.

ncbi gi num :

300193032

ncbi acc num :

NP_001177871.1

ncbi gb acc num :

NM_001190942.1

uniprot acc num :

P50591

ncbi pathways :

Apoptosis Pathway (198797); Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); Caspase-8 Activation Pathway (105654); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Death Receptor Signalling Pathway (105650); Dimerization Of Procaspase-8 Pathway (105655)

ncbi summary :

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

Function: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Cofactor: Binds 1 zinc ion per trimer. Subunit structure: Homotrimer. Subcellular location: Membrane; Single-pass type II membrane protein . Potential. Tissue specificity: Widespread; most predominant in spleen, lung and prostate. Sequence similarities: Belongs to the tumor necrosis factor family.

size1 :

0.1 mg

price1 :

475 USD

more info or order :

MyBioSource product webpage