antibody

Mouse Anti-Human DNAM-1

MBS690221

0.1 mg

475 USD

monoclonal

mouse

nonconjugated

(1X20)

Antibody

Mouse Anti-Human DNAM-1

DNAM-1

CD226; PTA1; DNAM1; DNAM-1; TLiSA1

CD226 antigen; CD226 antigen; CD226 antigen; adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; T lineage-specific activation antigen 1 antigen; CD226 molecule; DNAX accessory molecule 1

DNAM-1

CD226; CD226; PTA1; DNAM1; DNAM-1; TLiSA1; DNAM1; DNAM-1

CD226_HUMAN

Monoclonal

IgG1

(1X20)

Mouse

Human

336

Protein G chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Western Blot (WB), Neutr

Western Blot (WB): 1:500 - 1:1000. Neutralization of DNAM-1 bioactivity: Yes. This antibody was selected for its ability to detect human DNAM-1.

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant DNAM-1 extracellular domain. Antigen: Recombinant human DNAM-1 EC domain.

Reconstitution Buffer: PBS (Sterile). Reconstitution: Reconstitute the antibody with 200ul sterile PBS and the final concentration is 500 ug/ml.

DNAX accessory molecule1 (or DNAM1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Mature human DNAM1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2 set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, human DNAM1 shares 50% and 52% aa sequence identity with mouse and rat DNAM1, respectively. DNAM1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin2/ CD112. Ligation of DNAM1 promotes the activation of NK cells, CD8 + T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts. Platelet-endothelium interactions mediated by DNAM1 enable the metastasis of tumor cells to the lung. In activated, but not in resting NK, T, and mast cells, the cis association of DNAM1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM1 during activation.

171460997

NP_006557.2

NM_006566.2

Q15762

Adaptive Immune System Pathway (366160); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. [provided by RefSeq, Jul 2008]

Function: Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. Ref.1. Subunit structure: Interacts with PVR and PVRL2. Ref.7 Ref.8. Subcellular location: Membrane; Single-pass type I membrane protein . Potential. Tissue specificity: Expressed by peripheral blood T-lymphocytes. Ref.1. Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

0.1 mg

475 USD