antibody

Rat Anti-Mouse CCL-19

MBS690093

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[(10J38)]

Antibody

Rat Anti-Mouse CCL-19

[CCL-19]

[Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3]

[C-C motif chemokine 19; C-C motif chemokine 19; C-C motif chemokine 19; chemokine CCL19; EBI1 ligand chemokine; EBI1-ligand chemokine; EBI-1 ligand chemokine; small inducible cytokine A19; small-inducible cytokine A19; EBV-induced molecule 1 ligand chemokine; epstein-Barr virus-induced molecule 1 ligand chemokine; chemokine (C-C motif) ligand 19; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Small-inducible cytokine A19]

[CCL-19]

[Ccl19; Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3; Elc; Scya19; EBI1 ligand chemokine; ELC]

CCL19_MOUSE

Monoclonal

IgG2

[(10J38)]

Rat

Mouse

108

Protein G/A chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Ships at RT or 4 degree C.

Western Blot (WB), Neutr

WB: 1:500-1000. Neutralization: Yes

Prparartion: This antibody was produced from hybridoma (mouse myeloma fused with spleen cells from rat) immunized with mouse recombinant protein of CCL-19 (also known as MIP-3 Beta). Antigen: Recombinant Mouse CCL-19. Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Reconstruction Buffer: PBS sterile.

CCL19/MIP3beta, also known as ELC (EBI1Ligand Chemokine), is a beta chemokine that binds specifically to the chemokine receptor CCR7/EBI1/ BLR2. Mouse (human) CCL19 cDNA encodes a 108 (98) amino acid precursor protein with a predicted 25 (21) aa signal peptide that is cleaved to form the 83 (77) aa mature secreted protein. CCL19 is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse CCL19 shares 83% aa sequence homology with human CCL19. CCL19 has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of CCL19 is downregulated by the antiinflammatory cytokine IL10. Recombinant CCL19 has been shown to be chemotactic for Tcells and Bcells. The CCL19 receptor (CCR7/ EBI1/ BLR2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly upregulated in Bcells infected with EpsteinBarr virus and T-cells infected with herpesvirus 6 or 7.

6755422

NP_036018

NM_011888.2

O70460

11,911 Da

Chemokine Receptors Bind Chemokines Pathway (926993); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (926991); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (927022); GPCR Downstream Signaling Pathway (927020); GPCR Ligand Binding Pathway (926990)

Function: Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. Subcellular location: Secreted. Tissue specificity: Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus. Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

0.1 mg

490 USD