antibody

Rat Anti-Mouse CCL-17

MBS690090

0.1 mg

490 USD

monoclonal

rat

nonconjugated

[(9K49)]

western blot, neutralization

Antibody

Rat Anti-Mouse CCL-17

[CCL-17]

[Anti-mouse CCL-17TARC (#9K49)]

[chemokine (C-C motif) ligand 17; CC chemokine ABCD-2; chemokine (C-C motif) ligand 17; chemokine (C-C motif) ligand 17; small inducible cytokine subfamily A17; chemokine (C-C motif) ligand 17]

[CCL-17]

[Ccl17; Ccl17; Tarc; Abcd-2; Scya17; Scya17l; ABCD-2]

Q9WUZ6_MOUSE

Monoclonal

IgG2

[(9K49)]

Rat

Mouse

103

This antibody detects mouse CCL-17/TARC in western blotting and direct ELISA.

Protein A/G affinity chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity. Avoid repeated freezing and thawing cycles. Shipping: Ships with blue ice at 4°C.

Western Blot (WB), Neutralization

Western blot: 1:500-1000. Neutralization: Yes

Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse recombinant protein of CCL-17/TARC). Antigen: Recombinant mouse CCL-17

Reconstitution Buffer: PBS (sterile). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.

Human thymus and activation regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP1alpha, MIP1beta, MCP1, MCP2, MCP3, and I309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

225735579

NP_035462

NM_011332.3

Q9WUZ6

Chemokine Receptors Bind Chemokines Pathway (926993); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (926991); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (926990); Peptide Ligand-binding Receptors Pathway (926992); Signal Transduction Pathway (926897)

0.1 mg

490 USD