antibody

Mouse Anti-Human Pappalysin-2

MBS690059

0.1 mg

475 USD

monoclonal

mouse

nonconjugated

(8M12)

Antibody

Mouse Anti-Human Pappalysin-2

Pappalysin-2

PAPPA2; PAPPE; PLAC3; PAPP-A2

pappalysin-2 isoform 1; Pappalysin-2; pappalysin-2; placenta-specific 3; pregnancy-associated plasma protein E1; pregnancy-associated plasma preproprotein-A2; pappalysin 2; Pregnancy-associated plasma protein A2; PAPP-A2; Pregnancy-associated plasma protein E1

PAPPA2; PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2; PLAC3; PAPP-A2; PAPP-E

PAPP2_HUMAN

Monoclonal

IgG2

(8M12)

Mouse

Human

1791

Protein G chromatography

Lyophilized

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity. Shipping: Product ships on cold packs.

Western Blot (WB)

WB: Use at 1:500-1000

Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human Pappalysin-2, also called PAPP-A2.

Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. Antigen: Recombinant human Pappalysin-2. Remarks: This antibody was selected for its ability to detect human Pappalysin-2.

Pappalysins belong to a fifth family of metzincin proteases that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins. PAPPA is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the non-pregnant state. PAPPA is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPPA are significantly reduced when a fetus affected by Down syndrome is present. PAPPA cleaves Insulin-like Growth FactorBinding Protein4 and 5 (IGFBP4 and 5) at a single site, resulting in the release of bioactive IGF. Compared to PAPPA, PAPPA2 (also called PAPPE), the second member of the family is less characterized. PAPPA2 shares 45% amino acid identity to PAPPA in the mature form, which is synthesized as a preproprotein consisting of multiple domains. The prepro region (residues 1-233) and the C-terminal region (residues 1397-1791) are not included in recombinant human PAPPA2.

116174738

NP_064714.2

NM_020318.2

Q9BXP8

198,539 Da

Metabolism Of Proteins Pathway (106230); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Function: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Ref.1. Catalytic activity: Cleavage of the 143-Ser- -Lys-144 bond in insulin-like growth factor binding protein (IGFBP)-5. Cofactor: Binds 1 zinc ion per subunit . By similarity. Subunit structure: Monomer. Subcellular location: Secreted . By similarity. Tissue specificity: Expressed abundantly in placenta, and non-pregnant mammary gland with low expression in the kidney, fetal brain and pancreas. Ref.1 Ref.2. Sequence similarities: Belongs to the peptidase M43B family.Contains 5 Sushi (CCP/SCR) domains. Sequence caution: The sequence AAL17779.1 differs from that shown. Reason: Erroneous initiation. The sequence AAL17780.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC11134.1 differs from that shown. Reason: Erroneous initiation.

0.1 mg

475 USD