catalog number :
MBS689542
products full name :
Streptavidin HRP Conjugate (ELISA Grade)
products short name :
[Streptavidin HRP Conjugate]
form :
Lyophilized. 1.0 mg/ml of lyophilized antibody (E 1% at 280 nm = 32.0). (Product has been overfilled to ensure maximum recovery)
storage stability :
Store lyophilized material at 2-8°C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid. Prepare working dilution prior to use and then discard. Shelf life: 1 year from date of receipt.
tested application :
ELISA (EIA); Western Blot (WB); Immunohystochemistry (IHC); CL
app notes :
This conjugate is designed to be used in the detection of biotinylated primary antibodies. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s):. • 1:2,000-1:20,000 for Immunohistochemistry. • 1:1,000-1:50,000 for ELISA/Western Blot. Testing Results: . ELISA: Dilution giving A 450 reading of >= 1.0 when tested against Biotinylated antibody coated at 2ug/ml. Results: 1:359,000
other info1 :
Label: Horseradish Peroxidase (HRP). Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
. Country of Origin: USA
other info2 :
Preservative: 0.1% (v/v) Kathon CG (use of sodium azide will inhibit enzyme activity of horseradish peroxidase). Reconstitution: Rehydrate with 1.1 ml of deionized water. Allow reconstituted product to stand at least 30 minutes at room temperature prior to dilution. If necessary centrifuge to remove any particulates. Testing Results: Appearance: Reddish powder. Results: PASS
products categories :
ELISA & Western Blot Reagents