Anti-CD57 | MyBioSource MBS684292 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CD57

catalog :

MBS684292

quantity :

1 mL (Prediluted)

price :

175 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[E20-I]

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Poorly differntiated human neuroblastoma tissue stained with anti-CD57 monospecific clonal antibody. Formalin fixed, paraffin embedded human tissue (4um section) stained according to related datasheet.

product information

catalog number :

MBS684292

products type :

Antibody

products full name :

Anti-CD57

products short name :

[CD57]

other names :

[galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; beta-1,3-glucuronyltransferase 1; Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; GlcUAT-P]

products gene name :

[CD57]

other gene names :

[B3GAT1; B3GAT1; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP; GLCATP; GlcAT-P; GlcUAT-P]

uniprot entry name :

B3GA1_HUMAN

clonality :

Monoclonal

clone :

[E20-I]

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

334

storage stability :

Store +4°C

tested application :

Immunohistochemistry -Paraffin (IHC-P)

app notes :

Immunohistochemistry (IHC-P) Paraffin: 1:100 - 1:200

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Buffer: 20 mM Tris-HCl, pH 8.0. Stabilizer: 20 mg/ml BSA

other info2 :

Preservative: 0.05% NaN3. Immunogen: Peptide derived from C-terminal sequence of human CD57. Antibody recognizes the epitope between Lys316-Val332. Cellular localization: Golgi apparatus membrane. Positive Control: Brain tissue. Note: Centrifuge the vial before use. IHC-P Protocol: 1. Deparaffinize the section in 3 changes of xylene, 10 minutes each. 2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each. 3. Rinse in distilled water, 2 x 5 minutes. 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes. 5. Wash in distilled water, 2 x 5 minutes. 6. For antigen retrieval immerse the slide in citrate buffer, pH 6.0*, and incubate in water bath for 25-35 minutes at 96-98?C. 7. Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes. 8. Rinse in distilled water, 2 x 5 minutes. 9. Wash in PBS (buffer A), 2 x 5 minutes . 10. CONCENTRATED: . Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber. READY TO USE (RTU): . Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber. 11. Wash 3 x 5 minutes with buffer A. 12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested (Please Inquire). 13. Wash 3 x 5 minutes with buffer A. 14. Apply the chromogen (DAB), 1-3 minutes. 15. Wash in distilled water, 2 x 5 minutes. 16. Rinse in CuSO4.5H2O solution /0,90g NaCl + 0,50g CuSO4.5 H2O in 100ml . distilled water/ . 17. Wash in distilled water, 1 x 2 minutes. 18. Stain in hematoxylin for 5 minutes. 19. Wash in distilled water, 3 x 2 minutes. 20. Rinse in 37mM ammonium hydroxide solution. 21. Wash in distilled water, 1 x 2 minutes. 22. Mount the slide for observation. * Citrate Buffer (10mM Citric Acid, 0.05% Tween-20, pH 6.0):. Citric acid (anhydrous) ------------- 1.92 g; Distilled water ------------ 1000 ml . Mix to dissolve in 700 ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at 4ºC for longer storage.

ncbi gi num :

77695914

ncbi acc num :

NP_061114.2

ncbi gb acc num :

NM_018644.3

uniprot acc num :

Q9P2W7

ncbi mol weight :

38,256 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (1269981); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1269984); Glycosaminoglycan Metabolism Pathway (1269972); Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway (1269980); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957); Other Types Of O-glycan Biosynthesis Pathway (82978); Other Types Of O-glycan Biosynthesis Pathway (349); Chondroitin Sulfate Biosynthesis Pathway (545279); Chondroitin Sulfate Biosynthesis Pathway (545411)

ncbi summary :

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

B3GAT1: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Belongs to the glycosyltransferase 43 family. Protein type: Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; Transferase; Glycan Metabolism - heparan sulfate biosynthesis; EC 2.4.1.135. Chromosomal Location of Human Ortholog: 11q25. Cellular Component: Golgi membrane; integral to membrane. Molecular Function: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity; metal ion binding; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity. Biological Process: carbohydrate metabolic process; chondroitin sulfate metabolic process; chondroitin sulfate proteoglycan biosynthetic process; glycosaminoglycan metabolic process; protein amino acid glycosylation

size1 :

1 mL (Prediluted)

price1 :

175 USD

size2 :

0.04 mL (Concentrate)

price2 :

185

size3 :

0.1 mL (Concentrate)

price3 :

345

size4 :

7 mL (Prediluted)

price4 :

365

size5 :

0.2 mL (Concentrate)

price5 :

465

size6 :

15 mL (Prediluted)

price6 :

540

size7 :

0.5 mL (Concentrate)

price7 :

585

size8 :

1 mL (Concentrate)

price8 :

895

more info or order :

MyBioSource product webpage