catalog number :
MBS684274
products type :
Detection Kit
products full name :
DB Detection KIT - rabbit/mouse dual, HRP/DAB
products short name :
Detection KIT - rabbit/mouse dual, HRP/DAB
storage stability :
2-8 degree C
tested application :
Immunohistochemistry (IHC)
other info1 :
Content: The kit contains reagents sufficient for 250 tests. There are three separate bottles:. Bottle A: HRP RabbiUMouse 50 ml ready-to-use solution containing secondary antibody conjugated with HRP, recognizing specifically rabbit and/or mouse IgG. Presented in buffered solution containing stabilizing protein and preservative. Bottle B: Substrate Buffer 50 ml - buffered solution (ready to use) containing hydrogen peroxide and preservative. Bottle C: 2.5 ml - containing DAB+ Chromogen solution. All reagents (A, B, C) are stable for 24 months when stored at 2-8 degree C in dark. Application Protocol: Substrate working solution preparation: . Mix 1 ml Substrate Buffer (Bottle B) and 201-11 of DAB Chromogen solution (Bottle C). Mix thoroughly. The working solution should be stored at 2-8 'C and applied within 5 days. After incubating the section with primary antibody and appropriate washing procedure, carry out the following steps:. 1. Apply the secondary antibody (bottle A) to the section for 25-30 minutes. 2. Apply the substrate working solution to the section for 2x3 minutes
other info2 :
Regulatory Status: ISO 9001:2008, ISO 13485:2003, CE
products description :
The rabbit/mouse peroxidase (HRP)/DAB+, is a polymer based system designed for application in immunohistochemistry as a universal kit that can be used with prediluted and concentrated primary antibodies (produced in rabbit and/or mouse) from different manufacturers. It is a two-step detection kit; first the tissue is incubated with the diluted (ready to use) secondary antibody (detection system - detecting both, rabbit and/or mouse IgGs), followed by the incubation of the section with the DAB+ Chromogen solution.