antibody

Anti - PKC-gamma

MBS684151

0.1 ml Concentrate

530 USD

monoclonal

rabbit

nonconjugated

A12-H

human, mouse, rat

western blot, ELISA, immunocytochemistry, immunoprecipitation, enzyme immunoassay

Antibody

Anti - PKC-gamma

PKC-gamma

Anti - PKC-g

Protein kinase C gamma type; Protein kinase C gamma type; protein kinase C gamma type; protein kinase C, gamma

PKC-gamma

PRKCG; PRKCG; PKCC; PKCG; SCA14; PKC-gamma; PKCG; PKC-gamma

KPCG_HUMAN

Monoclonal

A12-H

Rabbit

Human, mouse, rat

697

Human, rat, mouse-tested

Store at -20 degree C

ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

WB - 1:2 000, ELISA - 1:5 000 - 1:20 000, ICC - 1:100 - 1:500

Immunogen: Peptide derived from the human PKC-gamma sequence. Storage Buffer: 20 mM Tris-HCI, pH 8.0. Stabilizer: 10 mg/ml BSA

Preservative: 0.05% NaN3. Regulatory Status: ISO 9001:2008, ISO 13485:2003, CE

462455

P05129.3

P05129

78,448 Da

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Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq, Jul 2008]

Function: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress . By similarity. Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Ref.6. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain. Enzyme regulation: Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-514 (activation loop of the kinase domain), Thr-655 (turn motif) and Thr-674 (hydrophobic region), need to be phosphorylated for its full activation. Subunit structure: Interacts with GRIA4 . By similarity. Interacts with CDCP1. Interacts with TP53INP1 and p53/TP53. Ref.5 Ref.6. Subcellular location: Cytoplasm . By similarity. Cytoplasm perinuclear region . By similarity. Cell membrane; Peripheral membrane protein . By similarity. Cell junction synapse synaptosome . By similarity. Cell projection dendrite . By similarity. Note: Translocates to synaptic membranes on stimulation . By similarity. Tissue specificity: Expressed in Purkinje cells of the cerebellar cortex. Ref.15. Post-translational modification: Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4 . By similarity.Ubiquitinated. Ref.10. Involvement in disease: Spinocerebellar ataxia 14 (SCA14) [MIM:605361]: Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.15. Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 C2 domain.Contains 2 phorbol-ester/DAG-type zinc fingers.Contains 1 protein kinase domain.

0.1 ml Concentrate

530 USD