Anti - EBV/LMP-1 | MyBioSource MBS684137 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti - EBV/LMP-1

catalog :

MBS684137

quantity :

1 mL (Prediluted)

price :

175 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[D24-G]

reactivity :

Epstein-Barr virus

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

image 3 :

product information

catalog number :

MBS684137

products type :

Antibody

products full name :

Anti - EBV/LMP-1

products short name :

[EBV/LMP-1]

other names :

[Latent membrane protein 1; Latent membrane protein 1; LMP1; hypothetical protein; Protein p63]

products gene name :

[EBV/LMP-1]

other gene names :

[LMP1; LMP1; LMP-1]

uniprot entry name :

LMP1_EBVB9

clonality :

Monoclonal

clone :

[D24-G]

host :

Rabbit

reactivity :

Human

sequence length :

386

specificity :

Human

storage stability :

Store at +4°C.

tested application :

Immunohistochemistry (IHC) - Formalin/Paraffin

app notes :

IHC-P. dilution: 1:100-1:200

image1 heading :

Testing Data

image2 heading :

Testing Data #2

image3 heading :

Testing Data #3

other info1 :

Immunogen: Peptide derived from the internal region of Epstein-Barr virus (EBV), Latent Membrane Protein-1 (LMP1). Antibody recognizes the epitope between Asp293 -Asp312. Buffer: 20 mM Tris-HCI, pH 8.0. Stabilizer: 20 mg/ml BSA. Preservative: 0.05% NaN 3 . Cellular localization: Nucleus

other info2 :

IHC-P PROTOCOL: 1.Deparaffinize the section in 3 changes of xylene, 5 minutes each. 2.Wash the section in 96%, 80% and 0% thyl alcohol for 10 minutes each. 3.Rinse in distilled water. 4.Block the endogenous peroxidase by incubating the tissuein 3% hydrogen peroxide (H2O2) for 10 minutes. 5.Wash in distilled water for 5 minutes. 6.For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in ater bath at 96°C for 40 minutes. (Alternatively adjust to your own protocol, keeping the required pH) . 7.Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes. 8.Rinse in distilled water. 9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes. 10.CONCENTRATED:Incubate the section with primary antibody at the dilution 1:100 -1:200 for 1 hour in the closed wet chamber. READY TO USE (RTU):Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber. 11.Wash twice 5 minutes with buffer A. 12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP -Peroxide -DAB). Micropolymer-HRP detection kit rabbit/mouse . 13.Wash twice 5 minutes with buffer A. 14.Apply the chromogen (DAB), 1 -3 minutes. 15.Wash in water for 10 minutes. 16.Stain in hematoxylin for 5 minutes. 17.Wash in water for 10 minutes. 18.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each. 19.Wash the section in 2 changes of xylene for 2 minutes each. 20.Mount the slide for observation. Citrate Buffer(10mM Citric Acid, 0.05% Tween-20, pH 6.0):. Citric acid (anhydrous) ----------1.92 g; Distilled water ---------1000 ml. Mix to dissolve in 700 ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4°C for longer storage. PRECAUTIONS: 1.-We strongly recommend to use Primary Antibody Diluent eventually alternative diluent containing protease free BSA at the concentrations ? 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided. 2.Centrifuge the vial before use. 3.Intended for professional In Vitro Diagnostic use in laboratories. 4.Do not use after expiration date stamped on vial label. 5.Avoid contamination of the reagent. 6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results. 7.The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous. 8.Disposal of waste material must be conducted in accordance with local regulations. 9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.

ncbi gi num :

126373

ncbi acc num :

P03230.1

uniprot acc num :

P03230

uniprot summary :

Function: Acts as a CD40 functional homolog to prevent apoptosis of infected B-lymphocytes and drive their proliferation. Functions as a constitutively active tumor necrosis factor receptor that induces the activation of several signaling pathways, including those of the nuclear factor-kappa-B family. LMP1 signaling leads to up-regulation of antiapoptotic proteins and provide growth signals in latently infected cells. It is a short-lived protein probably degraded by the proteasome. Subunit structure: Interacts (via PXQXT motif) with host tumor necrosis factor receptor-associated factor (TRAF) proteins TRAF1, TRAF2, TRAF3 and TRAF5. Subcellular location: Host cell membrane; Multi-pass membrane protein Ref.8. Domain: Two regions, C-terminal-activating region 1 (CTAR1) and CTAR2, have been identified within the cytoplasmic carboxy terminal domain that activates NF-kappa-B. Ref.7. Post-translational modification: Ubiquitinated on the N-terminus. Ref.8. Sequence similarities: Belongs to the herpesviridae LMP-1 family.

size1 :

1 mL (Prediluted)

price1 :

175 USD

size2 :

0.04 mL (Concentrate)

price2 :

185

size3 :

0.1 mL (Concentrate)

price3 :

370

size4 :

7 mL (Prediluted)

price4 :

400

size5 :

0.2 mL (Concentrate)

price5 :

505

size6 :

15 mL (Prediluted)

price6 :

590

size7 :

0.5 mL (Concentrate)

price7 :

635

size8 :

1 mL (Concentrate)

price8 :

985

more info or order :

MyBioSource product webpage