product summary
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company name :
MyBioSource
product type :
antibody
product name :
Purified anti-E. coli RNA Polymerase alpha Antibody
catalog :
MBS680053
quantity :
0.1 mg
price :
380 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[
4RA2]
reactivity :
Escherichia coli str. K-12 substr. MG1655
application :
western blot
more info or order :
image
image 1 :
Total cell lysates from HeLa (negative control, lane 1) and E. coli strain BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with 0.1 ug/ml (1:5000 dilution) of purified RNA Polymerase subunit alpha (clone 4RA2). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody at a 1:3000 dilution. Ponceau S red staining (bottom) was used to confirm equal protein loading. Lane M is the molecular weight ladder.
product information
catalog number :
MBS680053
products type :
Antibody
products full name :
Purified anti-E. coli RNA Polymerase alpha Antibody
products short name :
[RNA Polymerase alpha]
products name syn :
[RNA polymerase subunit alpha; Transcriptase subunit alpha; pez; phs; sez]
other names :
[RNA polymerase, alpha subunit; DNA-directed RNA polymerase subunit alpha; RNA polymerase, alpha subunit; RNA polymerase subunit alpha; Transcriptase subunit alpha]
other gene names :
[rpoA; rpoA; ECK3282; JW3257; pez; phs; sez; pez; phs; sez; RNAP subunit alpha]
uniprot entry name :
RPOA_ECOLI
clonality :
Monoclonal
isotype :
Mouse IgG1
clone :
[
4RA2]
host :
Mouse
reactivity :
E. coli, Widely among Gram-negative bacteria, some Gram-positive bacteria
sequence positions :
[209-329]
sequence length :
329
specificity :
RNA polymerase alpha (E. Coli RNA Polymerase)
form :
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
concentration :
0.5 mg/ml
storage stability :
The antibody solution should be stored undiluted between 2 degree C and 8 degree C.
tested application :
Western Blot (WB)
app notes :
Each lot of antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-1.0 ug/mL (1:500-1:5000). It is recommended that the reagent be titrated for optimal performance for each application.
image1 heading :
Western Blot
other info1 :
Preparation: The antibody was purified by affinity chromatography.
other info2 :
Structure: alpha is a 36 kD subunit of the E. coli RNA polymerase.
products description :
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.
products references :
1. Lesley SA and Burgess RR. 1989. Biochemistry 28:7728.
ncbi gi num :
16131174
ncbi acc num :
NP_417754
ncbi gb acc num :
NC_000913.2
uniprot acc num :
P0A7Z4
ncbi mol weight :
36,512 Da
ncbi pathways :
Metabolic Pathways (131985); Purine Metabolism Pathway (1022); Purine Metabolism Pathway (307); Pyrimidine Metabolism Pathway (1023); Pyrimidine Metabolism Pathway (309); RNA Polymerase Pathway (1121); RNA Polymerase Pathway (442); RNA Polymerase, Bacteria Pathway (405267); RNA Polymerase, Bacteria Pathway (468326)
ncbi summary :
HT_Cmplx11_Mem: [More information is available at EcoGene: EG10893]. RpoA is the subunit of the RNA polymerase core enzyme. [More information is available at EcoCyc: EG10893].
uniprot summary :
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. HAMAP-Rule MF_00059. Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00059. Subunit structure: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. Domain: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements. HAMAP-Rule MF_00059. Sequence similarities: Belongs to the RNA polymerase alpha chain family.
size1 :
0.1 mg
price1 :
380 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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