catalog number :
MBS680047
products full name :
mAb to RNA polymerase beta
products short name :
RNA polymerase beta
products name syn :
MAb to beta (beta) subunit of E.coli RNA polymerase; E. coli RNA Polymerase beta Monoclonal Antibody- Ascites; RNAP subunit beta; rpoB; groN; nitB; rif; ron; stl; stv; tabD; RNA polymerase subunit beta; Transcriptase subunit beta
reactivity :
Most Bacterial
specificity :
RNA polymerase beta (E. coli RNA Polymerase)
storage stability :
Store at -20 degree C, Avoid repeat freeze/thaw
tested application :
Western Blot (WB), ChIP, IAC, ELISA (EIA), Immunoprecipitation (IP)
app notes :
Immunoaffinity Chromatography: Yes - AS/PG. Immunoprecipitation (IP): Yes. Western Blot: Yes - dilute at 1:1000
other info1 :
Immunogen: Recombinant Protein. Reported Reactivity: Bordetella, Chlamydia, E Coli, Myxococcus, Salmonella, Vibrio. Cross Reactivity Note: Most bacterial RNAP including Bordetella, Chlamydia, E Coli, Myxococcus, Salmonella, Vibrio
other info2 :
Polyol-responsive: Yes. Reconstitution: Add 100 ul ddH2O to tube and mix to dissolve. Product Note: This mAb is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. *Cross reactivity and Applications data is per published literature and/or unpublished data
products description :
MAb to beta subunit of E.coli RNA polymerase. Beta is a 150 kDA subunit of the E.coli RNA polymerase. Beta is a 150kDa subunit of E. coli RNA Polymerase. beta (beta) is a 150kDa subunit of the E.coli RNA polymerase. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
products references :
Bergendahl, V., Thompson, N., Foley, K., Olson, B., and Burgess, R.R. (2003). A cross-reactive polyolresponsive monoclonal antibody useful for isolation of core RNA polymerase from many bacterial species. Protein Expression and Purification, 31, 155-160. Burgess, R.R. and Thompson, N. (2002). Advances in gentle immunoaffinity purification. Curr. Opin. Biotech. 13,304-308.