catalog number :
MBS680039
products full name :
mAb to NusA
products short name :
NusA
products name syn :
MAb to NusA subunit of E.coli RNA polymerase; E. coli RNA NusA Monoclonal Antibody; L factor; N utilization substance protein A; Transcription elongation protein nusA
other names :
transcription termination/antitermination L factor; Transcription termination/antitermination protein NusA; transcription termination/antitermination L factor; N utilization substance protein A; Transcription termination/antitermination L factor
products gene name :
NusA
other gene names :
nusA; nusA; ECK3158; JW3138
uniprot entry name :
NUSA_ECOLI
sequence positions :
343-494
specificity :
NusA (E. coli NusA)
storage stability :
Store at -20 degree C
tested application :
Western Blot (WB), ELISA (EIA)
app notes :
Western Blot: Yes - dilute at 1:1000
other info1 :
Immunogen: Recombinant Protein. Reported Reactivity: Salmonella typhimurium, Serratia marcescens. Cross Reactivity Note: Salmonella typhimurium, Serratia marcescens
other info2 :
Polyol-responsive: Yes. Reconstitution: Add 100ul of ddH2O to the tube containing antibody and mix to dissolve. Product Note: Does not react with B. cereus nor B. subtilis. *Cross reactivity and Applications data is per published literature and/or unpublished data.
products description :
MAb to NusA subunit of E.coli RNA polymerase. NusA is a 64 kDA component of the E. coli transcription machinery involved in elongation termination. NusA is a 64kDa component of E. coli transcription machinery involved in elongation termination. NusA is a 64kDa component of the E. coli transcription machinery involved in elongation termination. Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA.
ncbi gb acc num :
NC_000913.2
ncbi mol weight :
54,871 Da
ncbi summary :
Transcription termination/antitermination L factor (NusA) is a key component in both prevention and enhancement of transcriptional termination. [More information is available at EcoCyc: EG10665].
uniprot summary :
Function: Participates in both transcription termination and antitermination. Involved in a variety of cellular and viral termination and antitermination processes, such as Rho-dependent transcriptional termination, intrinsic termination, and phage lambda N-mediated transcriptional antitermination. Also important for coordinating the cellular responses to DNA damage by coupling the processes of nucleotide excision repair and translesion synthesis to transcription. Ref.8 Ref.9 Ref.10 Ref.13 Ref.15 Ref.17 Ref.18 Ref.20 Ref.21. Subunit structure: Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA. Also interacts with the termination Rho factor and the phage lambda N protein. Ref.7 Ref.8 Ref.11 Ref.14 Ref.15. Subcellular location: Cytoplasm. Note: Colocalizes with nucleoids. Ref.19. Induction: In response to low temperature. Negatively autoregulated. Ref.3 Ref.12. Domain: The N-terminal region interacts with RNA polymerase. The central region is composed of 3 RNA binding domains, S1, KH 1 and KH 2. The C-terminal region contains 2 acidic repeats, AR1 and AR2, which bind to protein N from phage lambda during antitermination. Ref.21 Ref.22 Ref.23. Disruption phenotype: Mutants are sensitive to DNA-damaging agents. Ref.20. Sequence similarities: Belongs to the NusA family.Contains 2 KH domains.Contains 1 S1 motif domain.