antibody

mAb to RNA polymerase alpha

MBS680032

0.1 mL

380 USD

monoclonal

mouse

nonconjugated

4RA2

western blot, ELISA, immunoprecipitation, enzyme immunoassay, chromatin immunoprecipitation

Antibody

mAb to RNA polymerase alpha

RNA polymerase alpha

MAb to alpha (alpha) subunit of E.coli RNA polymerase; E. coli RNA Polymerase alpha Monoclonal Antibody- Ascites; RNA polymerase subunit alpha; Transcriptase subunit alpha; rpoA; pez; phs; sez

RNA polymerase, alpha subunit; DNA-directed RNA polymerase subunit alpha; RNA polymerase, alpha subunit; RNA polymerase subunit alpha; Transcriptase subunit alpha

rpoA; rpoA; ECK3282; JW3257; pez; phs; sez; pez; phs; sez; RNAP subunit alpha

RPOA_ECOLI

Monoclonal

IgG1

4RA2

Mouse

Wide among gram negative bacteria, some gram positive bacteria

209-329

329

RNA polymerase alpha (E. Coli RNA Polymerase)

Ascites

Store at -20 degree C

Western Blot (WB), ChIP, IAC, ELISA (EIA), Immunoprecipitation (IP)

Immunoaffinity Chromatography: Yes - AS and 2,3-butandiol. Immunoprecipitation (IP): Yes. Western Blot: Yes - dilute at 1:1000

Immunogen: Recombinant Protein. Reported Reactivity: Widely among Gram-negative bacteria, some Gram-positive bacteria. Cross Reactivity Note: Widely among Gram-negative bacteria, some Grampositive bacteria

Polyol-responsive: Yes. Reconstitution: Add 100ul of ddH2O to the tube containing antibody and mix to dissolve. Product Note: Has been reported to purify RNAP from Pseudomonas aeruginosa *Cross reactivity and Applications data is per published literature and/or unpublished data.

MAb to alpha subunit of E.coli RNA polymerase. Alpha is a 36 kDA subunit of the E.coli RNA polymerase. alpha is a 36kDA subunit of E.coli RNA polymerase. alpha (alpha) is a 36kDA subunit of the E.coli RNA polymerase. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The alpha subunit of RNA polymerase plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.

16131174

NP_417754

NC_000913.2

P0A7Z4

36,512 Da

Metabolic Pathways (131985); Purine Metabolism Pathway (1022); Purine Metabolism Pathway (307); Pyrimidine Metabolism Pathway (1023); Pyrimidine Metabolism Pathway (309); RNA Polymerase Pathway (1121); RNA Polymerase Pathway (442); RNA Polymerase, Bacteria Pathway (405267); RNA Polymerase, Bacteria Pathway (468326)

HT_Cmplx11_Mem: [More information is available at EcoGene: EG10893]. RpoA is the subunit of the RNA polymerase core enzyme. [More information is available at EcoCyc: EG10893].

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. HAMAP-Rule MF_00059. Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00059. Subunit structure: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. Domain: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements. HAMAP-Rule MF_00059. Sequence similarities: Belongs to the RNA polymerase alpha chain family.

0.1 mL

380 USD