antibody

mAb to Sigma S

MBS680013

0.1 mL

380 USD

monoclonal

mouse

nonconjugated

1RS1

western blot, ELISA, enzyme immunoassay

Antibody

mAb to Sigma S

Sigma S

MAb to sigmaS (sigma S) subunit of E.coli RNA polymerase; E. coli RNA Sigma S Monoclonal Antibody; Sigma 38; rpoS; appR; katF; nur; otsX; sigS

RNA polymerase, sigma S (sigma 38) factor; RNA polymerase sigma factor RpoS; RNA polymerase, sigma S (sigma 38) factor; Sigma S; Sigma-38

Sigma S

rpoS; rpoS; abrD; appR; csi2; dpeB; ECK2736; JW5437; katF; nur; otsX; sigS; appR; katF; nur; otsX; sigS

RPOS_ECOLI

Monoclonal

IgG1

1RS1

Mouse

Most enterics

33-256

330

Sigma S (E. coli Sigma S (Strain MG1655))

Ascites

Store at -20 degree C

Western Blot (WB), ELISA (EIA), Transcription Inhibition

Transcription Inhibition - Initiation: Yes. Western Blot: Yes - dilute at 1:1000

Immunogen: Recombinant Protein. Reported Reactivity: E Coli, Klebsiella, Salmonella, Serratia, Bordetella and an unidentified protein at 60 kDA. Cross Reactivity Note: E Coli, Klebsiella, Salmonella, Serratia, Bordetella and an unidentified protein at 60 kDA

Polyol-responsive: No. Reconstitution: Add 100ul of ddH2O to the tube containing antibody and mix to dissolve. Product Note: Does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthrobacter, or Borrelia *Cross reactivity and Applications data is per published literature and/or unpublished data.

MAb to sigma S subunit of E.coli RNA polymerase. Sigma S is a 38 kDA subunit of the E.coli RNA polymerase. Sigma S is a 38kDa transcription factor of E. coli RNA Polymerase. sigmaS (sigma S) is a 38kDa subunit of the E.coli RNA polymerase. Sigma transcription factor controlling a regulation of genes required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon and other genes by modifying the affinity of RNA polymerase for the promoters.

16130648

NP_417221

NC_000913.2

P13445

37,972 Da

Polyamine modulon. [More information is available at EcoGene: EG10510]. rpoS encodes the alternative sigma factor S, a subunit of RNA polymerase that acts as the master regulator of the general stress response in Escherichia coli. [More information is available at EcoCyc: EG10510].

Function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management. Subunit structure: Interacts with the RNA polymerase core enzyme and RssB. Subcellular location: Cytoplasm . Potential. Induction: Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease. Sequence similarities: Belongs to the sigma-70 factor family. RpoS subfamily. Sequence caution: The sequence BAE76818.1 differs from that shown. Reason: Erroneous termination at position 33. Translated as Gln.The sequence CAA34435.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAA78692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.1 mL

380 USD