This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
antibody
product name :
mAb to Sigma 32
catalog :
MBS680012
quantity :
0.1 mg
price :
380 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3RH3
reactivity :
Escherichia coli str. K-12 substr. MG1655
application :
western blot, ELISA, enzyme immunoassay, chromatin immunoprecipitation
product information
catalog number :
MBS680012
products type :
Antibody
products full name :
mAb to Sigma 32
products short name :
Sigma 32
products name syn :
MAb to sigma32 (sigma 32) subunit of E.coli RNA polymerase; E. coli RNA Sigma 32 Monoclonal Antibody; SigmaH; rpoH; fam; hin; htpR; Heat shock regulatory protein F33.4
other names :
RNA polymerase, sigma 32 (sigma H) factor; RNA polymerase sigma-32 factor; RNA polymerase, sigma 32 (sigma H) factor; Heat shock regulatory protein F33.4
products gene name :
Sigma 32
other gene names :
rpoH; rpoH; ECK3445; fam; hin; htpR; JW3426; fam; hin; htpR
uniprot entry name :
RP32_ECOLI
clonality :
Monoclonal
isotype :
IgG1
clone :
3RH3
host :
Mouse
reactivity :
E Coli
sequence positions :
25-Jan
sequence length :
284
specificity :
Sigma 32 (E. coli Sigma 32)
purity :
Protein G purified
form :
Affinity Purified
concentration :
1 mg/ml in PBS pH 7.2
storage stability :
Store at -20 degree C lyophilized, 4 degree C Reconstituted, or sub-aliquot and store at -20 degree C
tested application :
Western Blot (WB), ChIP, ELISA (EIA), Transcription Inhibition
app notes :
Immunoprecipitation (IP): Yes, holoenzyme. Transcription Inhibition - Initiation: Yes, in vitro and S-30. Western Blot: Yes - dilute at 1:1000
other info1 :
Immunogen: Recombinant Protein
other info2 :
Reconstitution: Add 100ul of ddH2O to the tube containing antibody and mix to dissolve. Product Note: Cross reactivity and Applications data is per published literature and/or unpublished data
products description :
MAb to sigma 32 subunit of E.coli RNA polymerase. Sigma 32 is a 32 kDA subunit of the E.coli RNA polymerase. Sigma 32 is a 32kDa transcription factor of E.coli RNA polymerase. sigma32 (sigma 32) is a 32kDa subunit of the E.coli RNA polymerase. Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters.
ncbi gi num :
16131333
ncbi acc num :
NP_417918
ncbi gb acc num :
NC_000913.2
uniprot acc num :
P0AGB3
ncbi mol weight :
32,469 Da
ncbi summary :
RpoH is phosphorylated at Tyr-260, in HTH DNA-binding region 4.2, attenuating activity. de-phosphorylated by Etp. rpoH can be deleted, but null mutants do not grow above 20C (Zhou, 1988). [More information is available at EcoGene: EG10897]. Sigma 32 is the primary sigma factor controlling the heat shock response during log-phase growth. [More information is available at EcoCyc: EG10897].
uniprot summary :
Function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters. Sequence similarities: Belongs to the sigma-70 factor family. Sigma-32 subfamily.
size1 :
0.1 mg
price1 :
380 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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