mAb to RNA polymerase beta prime | MyBioSource MBS680007 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

mAb to RNA polymerase beta prime

catalog :

MBS680007

quantity :

0.1 mg

price :

380 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NT73

reactivity :

Escherichia coli str. K-12 substr. MG1655

application :

western blot, ELISA, immunoprecipitation, enzyme immunoassay, chromatin immunoprecipitation

product information

catalog number :

MBS680007

products type :

Antibody

products full name :

mAb to RNA polymerase beta prime

products short name :

RNA polymerase beta prime

products name syn :

MAb to beta' (beta prime) subunit of E.coli RNA polymerase; E. coli RNA Polymerase beta prime Monoclonal Antibody- Purified; RNA pol beta prime; rpoC; tabB; DNA-directed RNA polymerase subunit beta prime; RNAP subunit beta prime; RNA polymerase subunit beta prime; Transcriptase subunit beta prime

other names :

RNA polymerase, beta prime subunit; DNA-directed RNA polymerase subunit beta'; RNA polymerase, beta prime subunit; RNA polymerase subunit beta'; Transcriptase subunit beta'

other gene names :

rpoC; rpoC; ECK3979; JW3951; tabB; tabB; RNAP subunit beta'

uniprot entry name :

RPOC_ECOLI

clonality :

Monoclonal

isotype :

IgG1

clone :

NT73

host :

Mouse

reactivity :

E Coli

sequence positions :

1295-1417

sequence length :

1407

sequence :

SLAELLNAGLGGS

specificity :

RNA polymerase beta prime (E. Coli RNA Polymerase)

form :

Affinity Purified

concentration :

1 mg/ml in PBS pH 7.2

storage stability :

Store at -20 degree C lyophilized, 4 degree C Reconstituted, or sub-aliquot and store at -20 degree C

tested application :

Western Blot (WB), ChIP, IAC, ELISA (EIA), Immunoprecipitation (IP)

app notes :

Immunoaffinity Chromatography: Yes - AS/PG, NaCl/PG, AS/EG, NaCl/EG. Immunoprecipitation (IP): Yes. Western Blot: Yes - dilute at 1:1000

other info1 :

Immunogen: Recombinant Protein. Reported Reactivity: Enterics, Borellia burgdorferi, Salmonella. Cross Reactivity Note: Enterics, Borellia burgdorferi, Salmonella

other info2 :

Polyol-responsive: Yes. Reconstitution: Add 100ul of ddH2O to the tube containing antibody and mix to dissolve. Product Note: This mAb is extremely useful for purifying holoenzymes from enterics. It does not cross-react with mycobacterial M. smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems. *Cross reactivity and Applications data is per published literature and/or unpublished data

products description :

MAb to beta prime subunit of E.coli RNA polymerase. Beta prime is a 155 kDA subunit of the E.coli RNA polymerase. This antibody cross reacts with several prokaryotic RNA polymerases and recognizes Softag 1 epitope tagged proteins. Beta prime is a 155 kDA subunit of E.coli RNA polymerase. beta' (beta prime) is a 155kDa subunit of the E.coli RNA polymerase. RNA polymerase beta prime (rpoC) the beta prime subunit of the bacterial RNA polymerase, is a subunit which along with beta limits core polymerase expression.

products references :

Thompson, N. E., Hager, D. A., and Burgess R. R. (1992). Isolation and characterization of polyolresponsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase. Biochemistry 31, 7003-8. Thompson, N., Arthur T., and Burgess, R.R. (2003). Development of a new epitope tag for the gentle purification of proteins by immunoaffinity chromatography. Anal. Biochem. 323,171-179

ncbi gi num :

16131818

ncbi acc num :

NP_418415

ncbi gb acc num :

NC_000913.2

uniprot acc num :

P0A8T7

ncbi mol weight :

155,160 Da

ncbi pathways :

Metabolic Pathways 131985!!Purine Metabolism Pathway 1022!!Purine Metabolism Pathway 307!!Pyrimidine Metabolism Pathway 1023!!Pyrimidine Metabolism Pathway 309!!RNA Polymerase Pathway 1121!!RNA Polymerase Pathway 442!!RNA Polymerase, Bacteria Pathway 405267!!RNA Polymerase, Bacteria Pathway 468326

ncbi summary :

HT_Cmplx11_Mem: [More information is available at EcoGene: EG10895]. Along with its partner, the ' subunit of is integrally involved in the enzymatic function of RNA polymerase, especially at the promoter melting stage. [More information is available at EcoCyc: EG10895].

uniprot summary :

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP-Rule MF_01322. Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01322. Subunit structure: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription . By similarity. Sequence similarities: Belongs to the RNA polymerase beta' chain family.

size :

0.1 mg

price :

380 USD