catalog number :
MBS680001
products full name :
mAb to Sigma E
products short name :
Sigma E
products name syn :
MAb to sigmaE (sigma E) subunit of E.coli RNA polymerase; E. coli RNA Sigma E Monoclonal Antibody; rpoE; SigE; Sigma-24
other names :
RNA polymerase, sigma 24 (sigma E) factor; RNA polymerase sigma-E factor; RNA polymerase, sigma 24 (sigma E) factor; Sigma-24
products gene name :
Sigma E
other gene names :
rpoE; rpoE; ECK2571; JW2557; sigE; sigE
uniprot entry name :
RPOE_ECOLI
specificity :
Sigma E (E. coli Sigma E)
concentration :
1 mg/ml in PBS pH 7.2
storage stability :
Store at -20 degree C lyophilized, 4 degree C Reconstituted, or sub-aliquot and store at -20 degree C
tested application :
Western Blot (WB), ChIP, IAC, ELISA (EIA), Immunoprecipitation (IP)
app notes :
Immunoaffinity Chromatography: Yes, for overproduced Sigma-E. Immunoprecipitation (IP): Yes. Western Blot: Yes - dilute at 1:1000
other info1 :
Immunogen: Recombinant Protein. Reported Reactivity: Pseudomonas
other info2 :
Polyol-responsive: Yes. Reconstitution: Add 100ul of ddH2O to the tube containing lyophilized antibody and mix to dissolve
products description :
MAb to sigma E subunit of E.coli RNA polymerase. Sigma E) is a 24 kDA subunit of the E.coli RNA polymerase. Sigma E is a 24kDa transcription factor of E.coli RNA polymerase. sigmaE (sigma E) is a 24kDa subunit of the E.coli RNA polymerase. Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing in the extracytoplasmic compartment.
ncbi gb acc num :
NC_000913.2
ncbi mol weight :
21,696 Da
ncbi summary :
rpoE is transiently induced after a cold shock, dependent on the presence of PNPase. [More information is available at EcoGene: EG11897]. Sigma E is a minor sigma factor, specializing in responses to the effects of heat shock and other stresses on membrane and periplasmic proteins. [More information is available at EcoCyc: EG11897].
uniprot summary :
Function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing in the extracytoplasmic compartment. Induction: Transiently induced by cold shock in a PNPase-dependent fashion. Ref.11. Sequence similarities: Belongs to the sigma-70 factor family. ECF subfamily. Sequence caution: The sequence BAA10920.1 differs from that shown. Reason: Erroneous initiation.