catalog number :
MBS679088
products type :
Isotype Control
products full name :
Rat IgG2a-UNLB
products short name :
Rat IgG2a Isotype Control
products name syn :
Rat IgG2a - Purified, Unlabeled Antibody; Rat IgG2a Isotype Control
isotype :
Rat IgG2a kappa
specificity :
Keyhole limpet hemocyanin (KLH)
form :
Purified (UNLB) Antibody
storage stability :
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The Alexa Fluor 488 (AF488) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1mg in 0.2mL PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!. The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
tested application :
ELISA; Flow Cytometry
app notes :
WORKING DILUTIONS . Flow Cytometry . Flourescein, AF488, and PacBlu conjugates: < or = ug/10^6 cells. Biotin conjugate: < or = ug/10^6 cells. R-phycoerythrin conjugate: < or = 0.2 ug/10^6 cells. R-phycoerythrin conjugate-Texas Red^(R): < or = 0.2 ug/10^6 cells. Allophycocyanin conjugate: < or = 0.2 ug/10^6 cells. Spectral Red^TM conjugate: < or = 0.2 ug/10^6 cells. Cyanine 5 conjugate: < or = 0.2 ug/10^6 cells. PE/CY5.5 and PE/CY7 conjugates: < or = 0.2 ug/10^6 cells. APC/CY5.5 and APC/CY7 conjugates: < or = 0.2 ug/10^6 cells. AF647 conjugate: < or = 0.2 ug/10^6 cells.
other info1 :
Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium Azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
other info2 :
Characterization: To insure lot-to-lot consistency, each batch of product is tested to conform with characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.
products categories :
Rat IgG2a kappa Isotype Control