secondary antibody

Rabbit Anti-Mouse IgG (H+L)-HRP

MBS674902

1 mL

155 USD

Secondary Antibody

Rabbit Anti-Mouse IgG (H+L)-HRP

Rabbit Anti-Mouse IgG (H+L)

Rabbit Anti-Mouse IgG(H+L) - HRP; Rabbit Anti-Mouse IgG (H+L)

Polyclonal

IgG

Rabbit

Mouse

Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA and with the light chains of mouse IgM and IgA as demonstrated by ELISA or FLISA; may react with other species.

Affinity chromatography on pooled mouse IgG covalently linked to agarose.

Horseradish Peroxidase (HRP) Conjugate

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot; Flow Cytometry; FLISA; Immunoprecipitation

FLISA:. FITC conjugate: 1:200-1:400. ELISA: . AP conjugate: 1:2,000-1:4,000. HRP conjugate: 1:4,000-1:8,000. BIOT conjugate: 1:5,000-1:20,000. Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Source: Pooled antisera from rabbits hyperimmunized with mouse IgG paraproteins. Cross Absorption: NA

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin eyes, or cloting. Wear eye of face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Rabbit Anti-Mouse IgG (H+L)

1 mL

155 USD