secondary antibody

Rabbit F(ab')2 Anti-Human IgG (H+L), Mouse ads-HRP

MBS674781

1 mL

225 USD

Secondary Antibody

Rabbit F(ab')2 Anti-Human IgG (H+L), Mouse ads-HRP

Rabbit F(ab')2 Anti-Human IgG (H+L)

Rabbit F(ab')2 Anti-Human IgG(H+L), Mouse ads - HRP; Rabbit F(ab')2 Anti-Human IgG (H+L) (Absorbed against mouse immunoglobulins)

Polyclonal

IgG

Rabbit

Human

Reacts with the heavy and light chains of human IgG as demonstrated by ELISA.May also react with the light chains of other human immunoglobulins. Minimal cross reactivity with mouse immunoglobulins.

Affinity chromatography on human IgG covalently coupled to agarose. Gel filtration chromatography of pepsin digested antibody.

Horseradish Peroxidase (HRP) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot

ELISA Suggested Dilution: 1:4,000-1:8,000

Source: Pepsin digest of rabbit anti-human IgG (H+L). Cross Absorption: Pooled human immunoglobulins.

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA and/or Fluorescent ELISA for conformance to characteristics of a standard reference reagent.

Rabbit F(ab')2 Anti-Human IgG (H+L)

1 mL

225 USD