secondary antibody

Goat Anti-Rabbit IgG (H+L), Mouse/Human ads-HRP

MBS674754

1 mL

170 USD

Secondary Antibody

Goat Anti-Rabbit IgG (H+L), Mouse/Human ads-HRP

Goat Anti-Rabbit IgG (H+L chain specific)

Goat Anti-Rabbit IgG(H+L), Mouse/Human ads - HRP; Goat Anti-Rabbit IgG (H+L chain specific) (Absorbed against human & mouse immunoglobulins)

Polyclonal

IgG

Goat

Rabbit

Reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human and mouse immunoglobulins.

Affinity chromatography on pooled IgG covalently linked to agarose.

Horseradish Peroxidase (HRP) Conjugate

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The beta -galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS/NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry

ELISA Suggested Dilution: 1:4,000-1:8,000

Source: Pooled antisera from goats hyperimmunized with normal rabbit IgG. Cross Absorption: Pooled human sera, purified human paraproteins, pooled mouse sera, and pooled mouse plasmacytoma/hybridoma proteins.

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Goat Anti-Rabbit IgG (H+L chain specific)

1 mL

170 USD