secondary antibody

Goat F(ab')2 Anti-Rat IgG (H+L), Mouse ads-LE/AF

MBS674723

0.5 mg

310 USD

Secondary Antibody

Goat F(ab')2 Anti-Rat IgG (H+L), Mouse ads-LE/AF

Goat F(ab')2 Anti-Rat IgG (H+L chain specific)

Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads - Low Endotoxin/Azide-Free; Goat F(ab')2 Anti-Rat IgG (H+L chain specific) (Absorbed against mouse immunoglobulins)

Polyclonal

IgG

Goat

Rat

Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with mouse immunoglobulins.

Gel filtration of pepsin-digested antibody.

Low Endotoxin, Azide - Free (LE/AF)

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) and Texas Red (TXRD) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD), R-phycoerythrin-Texas Red (R-PE-TXRD), and R-phycoerythrin- Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Direct and Indirect Immunofluorescent Staining; ELISA; FLISA; Immunoblot; Immunohistochemistry

Working Dilutions:. Flow Cytometry: . Purified (UNLB) antibody: < or = 1ug/10^6 cells. FITC and BIOT conjugates: < or = 1ug/10^6 cells. PE, PE/TXRD, SPRD, and PE/CY5.5 conjugates: < or = 0.1ug/10^6 cells. FLISA:. TXRD conjugates: 1:100-1:400. ELISA: . BIOT conjugates: 1:5,000-1:20,000. Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Source: Pepsin digest of goat anti-rat IgG (H+L). Cross Absorption: Pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry for conform to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Goat F(ab')2 Anti-Rat IgG (H+L chain specific)

0.5 mg

310 USD