secondary antibody

Goat Anti-Rat IgG-HRP

MBS674702

1 mL

170 USD

Secondary Antibody

Goat Anti-Rat IgG-HRP

Goat Anti-Rat IgG (gamma chain specific)

Goat Anti-Rat IgG - HRP; Goat Anti-Rat IgG (gamma chain specific)

Polyclonal

IgG

Goat

Rat

Reacts with the heavy chain of rat IgG as demonstrated by ELISA or FLISA. May also react with IgG from other species.

Affinity chromatography on rat IgG covalently linked to agarose

Horseradish Peroxidase (HRP) Conjugate

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) and rhodamine (TRITC) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The beta -galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS/NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Direct Immunofluorescent Staining of IgG* Rat B Lymphocytes. ELISA. FLISA. Immunoblotting. Immunohistochemistry

WORKING DILUTIONS: . FLISA: . FITC conjugate: 1:200-1:400. TRITC conjugate: 1:100-1:400. PE conjugate: 1ug/mL. ELISA. AP conjugate: 1:2,000-1:4,000. HRP conjugate: 1:4,000-1:8,000. BGAL conjugate: 1:500. HRP conjugate: 1:5,000-1:20,000. Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Source: Pooled antisera from goats hyperimmunized with normal rat IgG. Cross Absorption: Rat IgM

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Goat Anti-Rat IgG (? chain specific)

1 mL

170 USD