secondary antibody

Goat Anti-Rat IgM-HRP

MBS674694

1 mL

175 USD

Secondary Antibody

Goat Anti-Rat IgM-HRP

Goat Anti-Rat IgM (u chain specific)

Goat Anti-Rat IgM - HRP; Goat Anti-Rat IgM (u chain specific)

Polyclonal

IgG

Goat

Rat

Reacts with the heavy chain of rat IgM as demonstrated by ELISA or FLISA. May also react with IgM from other species.

Affinity chromatography on rat IgM covalently linked to agarose

Horseradish Peroxidase (HRP) Conjugate

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Direct Immunofluorescent Staining of IgG* Rat B Lymphocytes. ELISA. FLISA. Immunoblotting. Immunohistochemistry

WORKING DILUTIONS: . FLISA: . FITC conjugate: 1:200-1:400. TRITC and TXRD conjugate: 1:100-1:400. PE conjugate: 1ug/mL. ELISA. AP conjugate: 1:2,000-1:4,000. HRP conjugate: 1:4,000-1:8,000. BIOT conjugate: 1:5,000-1:20,000. Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Source: Pooled antisera from goats hyperimmunized with normal rat IgM. Cross Absorption: Rat IgG

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxicif ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If ingested or inhaled, contact a physician immediately. Sodium azide yileds toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid acculation of potenially explosive deposits in lead or copper plumbing.

Goat Anti-Rat IgM (u chain specific)

1 mL

175 USD