secondary antibody

Goat F(ab')2 Anti-Human Kappa, Mouse ads-BIOT

MBS674657

0.5 mg

320 USD

Secondary Antibody

Goat F(ab')2 Anti-Human Kappa, Mouse ads-BIOT

[Goat F(ab')2 Anti-Human kappa (kappa chain specific)]

[Goat F(ab')2 Anti-Human Kappa, Mouse ads - Biotin; Goat F(ab')2 Anti-Human kappa (kappa chain specific) (Adsorbed against mouse Igs)]

Polyclonal

IgG

Goat

Human

Reacts with human kappa light chains as demonstrated by ELISA, FLISA, and flow cytometry

Affinity chromatography on pooled human Igs with kappa light chains covalently linked to agarose.

Biotin (BIOT) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot

Working Dilutions:. Flow Cytometry. FITC and BIOT conjugates: < or = 1ug/10^6 cells. PE conjugate: < or = 0.1ug/10^6 cells. ELISA:. BIOT conjugate: 1:5,000-1:20,000. AP conjugate: 1:2,000-1:4,000. Other Applications: Since application vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Source: Pooled antisera from goats hyperimmunized with human myeloma proteins with kappa light chains. Cross Absorption: Pooled human myeloma proteins with lambda light chains and mouse immunoglobulins

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yeilds toxic hyrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Goat F(ab')2 Anti-Human kappa (kappa chain specific)

0.5 mg

320 USD