secondary antibody

Goat Anti-Human IgG-FITC

MBS674598

1 mg

165 USD

Secondary Antibody

Goat Anti-Human IgG-FITC

Goat Anti-Human IgG (gamma chain specific)

Goat Anti-Human IgG - FITC; Goat Anti-Human IgG (gamma chain specific)

Polyclonal

IgG

Goat

Human

Reacts with the heavy chain of human IgG as demonstrated by ELISA and flow cytometry. May also react IgG from other species.

Affinity chromatography on pooled human IgG covalently linked to agarose.

Fluorescein (FITC) Conjugate

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The beta -galactosidase (BGAL) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS/NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

ELISA; Immunoblot; Immunofluorescence

Research Application(s): Direct immunofluorescent staining of IgG+ human B lymphocytes. Immunofluorescence Suggested Dilution: <= 1 ug/10^6 cells

Source: Pooled antisera from goats hyperimmunized with normal human IgG and human IgG paraproteins. Cross Absorption: Human IgM, IgD and IgA.

Characterization: To ensure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent.

Goat Anti-Human IgG (? chain specific)

1 mg

165 USD