secondary antibody

Goat Anti-Mouse IgG2b, Human ads-APC

MBS674499

0.25 mg

315 USD

Secondary Antibody

Goat Anti-Mouse IgG2b, Human ads-APC

Goat Anti-Mouse IgG2b (gamma 2b chain specific)

Goat Anti-Mouse IgG2b, Human ads - Allophycocyanin; Goat Anti-Mouse IgG2b (gamma 2b chain specific)

Polyclonal

IgG

Pooled antisera from goates hyperimmunized with mouse IgG2b paraproteins

Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins.

Reacts with the heavy chain of mouse IgG2b as demonstrated by ELISA, FLISA, and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Affinity chromatography on mouse IgG2b covalently linked to agarose

Allophycocyanin (APC) Conjugate

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% 50 mM Tris, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

ELISA; FLISA; Immunoblotting; Indirect Immunostaining in conjunction with mouse IgG2b monoclonal antibodies

WORKING DILUTIONS . Immunofluorescence: APC conjugates: <= 0.1 ug/10^6 cells

Source: Pooled antisera from goats hyperimmunized with mouse IgG2b paraproteins. Cross Absorption: Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified human paraproteins.

Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent. Representative data are included in this product insert.

Goat Anti-Mouse IgG2b (?2b chain specific)

0.25 mg

315 USD