catalog number :
MBS674474
products type :
Secondary Antibody
products full name :
Goat Anti-Mouse IgG2a, Human ads-HRP
products short name :
Goat Anti-Mouse IgG2a (gamma 2a chain specific)
products name syn :
Goat Anti-Mouse IgG2a, Human ads - HRP; Goat Anti-Mouse IgG2a (gamma 2a chain specific)
specificity :
Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
purity :
Affinity chromatography on mouse IgG2a covalently linked to agarose.
form :
Horseradish Peroxidase (HRP) Conjugate
storage stability :
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
tested application :
Flow Cytometry (FC/FACS), FLISA, ELISA (EIA)
app notes :
Flow Cytometry: FITC, AF488, and BIOT conjugates FLISA: TRITC, TXRD and AF555 conjugates 1:100-1:400. ELISA: AP conjugate 1:2000-1:4000. HRP conjugate 1:4000-1:8000. BIOT conjugate 1:5000-1:20000. Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
other info1 :
Source: Pooled antisera from goats hyperimmunized with mouse IgG2a paraproteins. Cross Absorption: Mouse IgM, IgG1, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.
other info2 :
Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance with characteristics of a standard reference reagent.
products categories :
Goat Anti-Mouse IgG2a (?2a chain specific)