secondary antibody

Goat F(ab')2 Anti-Mouse Ig, Human ads-PE

MBS674346

0.25 mg

265 USD

Secondary Antibody

Goat F(ab')2 Anti-Mouse Ig, Human ads-PE

[Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L)]

[Goat F(ab')2 Anti-Mouse Ig, Human ads - Phycoerythrin-Labeled; Goat F(ab')2 Anti-Mouse Ig M+IgG+IgA (H+L)]

Polyclonal

Ig

Pepsin digest of goat anti-mouse Ig

Mouse

Reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b IgG3, and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.

Gel filtration chromatography of pepsin digested antibody.

R - phycoerythrin (R - PE) Conjugate

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot

Working Dilutions: . PE conjugates: <= 0.1 ug/10^6 cells. Other Applications: Since applications vary, you should determine the optimum working dilution of the product that is appropriate for you specificity need.

Source: Pepsin digest of goat anti-mouse Ig (H+L). Cross Absorption: Pooled human sera and purified human paraproteins.

Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry for conformance with characteristics of a standard reference reagent. Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hyrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Goat F(ab')2 Anti-Mouse IgM+IgG+IgA (H+L)

0.25 mg

265 USD