secondary antibody

Mouse Anti-Human CD79b/B29-BIOT

MBS674288

100 Tests

240 USD

Secondary Antibody

Mouse Anti-Human CD79b/B29-BIOT

CD79 beta (B29, Ig beta)

Mouse Anti-Human CD79b/B29 - Biotin; Mouse Anti-Human CD79 beta (B29, Ig beta)

Monoclonal

Mouse IgG1

CB3-1

Mouse

Human

Human CD79 beta /B29/Ig beta . CD79 is a 23/21 disulfide-linked heterodimer composed of an alpha chain (CD79a/mb-1) and a beta chain (CD79b/B29) that associates non-covalently with membrane immunoglobulin to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes, first appearing on the surface at the pro-B cell stage and remaining through all stages of B cell differentiation prior to plasma cells. Crosslinking of the BCR leads to B cell activation. The monoclonal antibody CB3-1 reacts with the beta chain of CD79.1-4

Biotin (BIOT) Conjugate

The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . The Spectral Red? (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! . Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation

Flow Cytometry Suggested Dilution: 10 uL/10^6 cells

Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of a standard reference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flow cytometer.

1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Kishimoto, T., A.E.G. von dern Borne, S.M. Goyert, D.Y. Mason, M. Miyasaka, L. Moretta, K. Okumura, S. Shaw, T.A. Springer, K. Sugamura, and H. Zola, eds. 1998. Leukocyte Typing VI: White Cell Differentiation Antigens, Academic Press, New York. 3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD79 Section, Academic Press, New York, p. 332. 4. Nakamura, T., H. Kubagawa, and M.D. Cooper, 1992. Proc. Natl. Acad. Sci. USA 89:8522.

100 Tests

240 USD